-
Plasmid#69456PurposeRNA-IN S4 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S4 + SFGFP
UseSynthetic BiologyTagsExpressionBacterialMutationmutations for S4 variant (from wt)PromoterJ23119 and J23119 (sigma 70)Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS3
Plasmid#69455PurposeRNA-IN S3 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S3
UseSynthetic BiologyTagsExpressionBacterialMutationmutations for S3 variant (from wt)PromoterJ23119 and J23119 (sigma 70)Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA5woH
Plasmid#65019PurposeExpresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureusDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, D160N, D165A, K19…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA2woH
Plasmid#65018PurposeExpresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108APromotertetracycline promoter/operatorAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSALECT-SupH
Plasmid#59710PurposepSALECT with SupH insertDepositorInsertSupH
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSALECT-AhyI
Plasmid#59708PurposepSALECT with AhyI insertDepositorInsertAhyI
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-D91G/V220A
Plasmid#47648PurposepAC-EsaR with a272g and c659t mutations in esaRDepositorInsertesaR-D91G/V220A
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Aspartic acid 91 to Glycine, and Valine 2…PromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSCT-GAL93-DEST
Plasmid#46339DepositorTypeEmpty backboneUseTags6XHISExpressionMammalianMutationPromoterpCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNH066
Plasmid#42162DepositorInsertN-TAP Tag-RT-::mTFP1
UseTagsTAP-TAgExpressionMutationPromoterAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNH094
Plasmid#42167DepositorInsertmTFP1-RT-::[(G4S)3]::C-TAP Tag
UseTagsExpressionMutationPromoterAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNH090
Plasmid#42166DepositorInsertmTFP1::[(G4S)3]::C-TAP Tag
UseTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJK384.4
Plasmid#155369PurposedCas9 + PA4-mVenusDepositorInsertsdCas9 (bacteria)
PA4-mVenus
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pJK412
Plasmid#155371PurposedCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]DepositorInsertsdCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCKTRBS-CbnR-ABE44898-OD
Plasmid#102925PurposepCKTRBS-OD derivative carrying CbnR-ABE44898-OD chimera, also known as ChBz01DepositorInsertCbnR-ABE44898-OD (ChTFBz01)
UseTagsExpressionMutationPromoterPtetOAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAW
Plasmid#133893PurposeExpression of Gal4AD-Prey hybrid proteinDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pBW
Plasmid#133897PurposeExpression of Gal4BD-Bait hybrid protein.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
2Flag-pDEST-C
Plasmid#118372PurposeGateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2FlagExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
2HA-pDEST-C
Plasmid#118374PurposeGateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2HAExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c_ddCpf1_final
Plasmid#212040PurposeBacterial expression of the CRISPRi system for genome editing in E. coliDepositorInsertddCpf1
UseCRISPRTagsExpressionMutationPromoterL-arabinose-inducible promoter PBADAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCasSA
Plasmid#98211PurposeCRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only