Addgene

We narrowed to 4,616 results for: LucY

Showing: 4381 - 4400 of 4616 results

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pHCKan-T7-CBD-hGLY

Plasmid

#134940

Purpose

T7 based expression of human glycine acyltransferase with an N-terminal chitin binding domain

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

human glycine acyltransferase 2 (GLYATL2 )

Use

Tags

chitin binding domain

Expression

Bacterial

Mutation

Promoter

t7

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pJOG659

Plasmid

#105410

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Luciferase CDS fused to 3xHA tag

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSMART-ala13

Plasmid

#87174

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Use

Tags

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proD

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pSMART-ala11

Plasmid

#87172

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Use

Tags

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proA

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSMART-ala12

Plasmid

#87173

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Use

Tags

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proC

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly-x16

Plasmid

#71505

Purpose

flySTARR-seq screening vector with x16 core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Mutation

Promoter

Available since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_LAMP1

Plasmid

#65685

Purpose

Expresses LAMP1-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

LAMP1

Use

Luciferase

Tags

CNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pYNL-N1_LAMP1

Plasmid

#65684

Purpose

Expresses LAMP1-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

LAMP1

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pSTARR-seq_fly-eEF1delta

Plasmid

#71501

Purpose

flySTARR-seq screening vector with eEF1delta core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Mutation

Promoter

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pYNL-N1_PTS2

Plasmid

#65675

Purpose

Expresses PTS2-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 2

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pTH729-minRLuc

Plasmid

#38225

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

Renilla Luciferase

Use

Tags

Expression

Yeast

Mutation

Every codon of the Renilla coding sequence was ex…

Promoter

TDH3 (=GPD)

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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PB-fLuc=tdT

Plasmid

#120870

Purpose

Piggybac transposon plasmid for live animal optical imaging

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

pcDNA3-fLuc=tdT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX_TRE3G_DD::mCherry

Plasmid

#108679

Purpose

Tunable system for controlled gene expression and protein stability in mammalian cells

Depositor

Lucia Marucci

Article

Pedone et al Nat Commun. 2019 Oct 2;10(1):4481. doi:

Insert

Destabilizing Domain (DD) mCherry fusion protein

Use

Lentiviral

Tags

DD::mCherry fusion protein

Expression

Mammalian

Mutation

Promoter

Doxycycline inducible

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBMN(CMV-copGFP-Luc2-Puro)

Plasmid

#80389

Purpose

To make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection marker

Depositor

Magnus Essand

Article

Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

Insert

copGFP-T2A-Luc2-F2A-Puromycin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE NFKB-TA-LUC-UBC-dTomato-W

Plasmid

#49335

Purpose

pHAGE NFKB-TA-LUC-UBC-dTomato-W

Depositor

Darrell Kotton

Article

Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

Inserts

NFkB consensus binding sequence
TA
Luciferase
UBC
dTomato

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_SCP1 vector_blocking 4

Plasmid

#99319

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas5

Plasmid

#178878

Purpose

Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas5c-bGH polyA

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas8

Plasmid

#178880

Purpose

Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas8c-bGH PolyA

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas7

Plasmid

#178879

Purpose

Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas7c-bGH PolyA

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSMART-sgRNA (Sp)

Plasmid

#80427

Purpose

U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

Depositor

Sara Howden, Melissa Little

Article

Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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