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Plasmid#169002PurposeExpress zebrafish Dnase1l1l-mRFP in zebrafishDepositorInsertDnase1l1l-mRFP (dnase1l3l Zebrafish)
UseUnspecifiedTagsmRFPExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCRII HSPB7
Plasmid#61034PurposeProbe for HSPB7 for in situ hybridization in zebrafishDepositorInsertHSPB7 (hspb7 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT22gcfUbm5En25EE4PCh(#552)
Plasmid#184092Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2(5E)-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutationchkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22Ubm4Eng2bKRPC(#493)
Plasmid#184091Purposecyclofen-inducible fish Eng2b (mut) activation in zebrafish permanent transgenicDepositorInsert4myc-ZfEng2b(WW>KK)-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags4xMycExpressionMutationZfEng2b: W141K, W144K; ERT2: G400V, M543A, L544A,…PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol2-nbt300 2n-KalNFB-3xmir1T-syt2a-10U2n-mCardinal-P2A-mClover3
Plasmid#196117Purposezebrafish expressionDepositorInsertnbt300 2n-KalNFB-3xmir1T-syt2a-HO1-10U2n-mCardinal-P2A-mClover3
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-F-actin-microtubule plus ends reporters
Plasmid#196134Purposezebrafish expressionDepositorInsertF-actin-microtubule plus ends reporters
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-epNTR
Plasmid#62213PurposeepNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2DepositorInsertepNTR
UseMrna synthesisTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2
Plasmid#74010PurposeTissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoterDepositorInserts5xUAS
Cas9
Cre
U6 promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-sod1
Plasmid#107594PurposeContains zebrafish sod1 gene in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertsod1 (sod1 Zebrafish)
UseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn3
Plasmid#164959PurposeZebrafish kcnn3 gene CDSDepositorInsertkcnn3 (kcnn3 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn4
Plasmid#164960PurposeZebrafish kcnn4 gene CDSDepositorInsertkcnn4 (kcnn4 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSws1-nfsB-CFP
Plasmid#86375Purposefor zebrafish transgenesis, expression of nfsB-CFP, also expresses EGFPDepositorInsertnitroreductase (nfsB bacteria)
UseZebrafishTagsECFPExpressionMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2-BiNLS-fYap
Plasmid#176623Purposeexpresses optofYap in zebrafishDepositorInsertYes associated protein (yap1 Zebrafish)
UseZebrafish expressionTagsmCherryExpressionMutationPromoterSP6Available sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZE43 Dapper-2 pCS2P+
Plasmid#17084DepositorInsertDapper-2 (dact2 Zebrafish)
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
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ZE46 DeltaPGB Dvl2 pCS2P+
Plasmid#17087DepositorInsertDvl-2 (dvl2 Zebrafish)
UseZebrafish expressionTagsExpressionMutationDeltaPGB (deletes amino acids 231-252)PromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
mhc2dab:GFP-LT
Plasmid#68996Purposefor making transgenic fishDepositorInsertmhc2dab (mhc2dab Zebrafish)
UseZebrafish expressionTagsGFP-LTExpressionMutationPromotermhc2dabAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnt2a
Plasmid#164963PurposeZebrafish kcnt2a gene CDSDepositorInsertkcnt2a (kcnt2 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnma1a
Plasmid#164951PurposeZebrafish kcnma1a gene CDSDepositorInsertkcnma1a (kcnma1a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only