Addgene

We narrowed to 3,077 results for: zebrafish

Showing: 421 - 440 of 3077 results

Results per page:

pENTR-D-TOPO-kcnn1b

Plasmid

#164957

Purpose

Zebrafish kcnn1b gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnn1b (kcnn1b Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCS2-SauCas9

Plasmid

#174040

Purpose

In vitro transcription of SauCas9 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

SauCas9

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT22Ubm4Eng2bKRPC(#493)

Plasmid

#184091

Purpose

cyclofen-inducible fish Eng2b (mut) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

Insert

4myc-ZfEng2b(WW>KK)-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

4xMyc

Expression

Mutation

ZfEng2b: W141K, W144K; ERT2: G400V, M543A, L544A,…

Promoter

Zf-Ubi

Availability

Academic Institutions and Nonprofits only

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pT22gcfUbm5En25EE4PCh(#552)

Plasmid

#184092

Purpose

cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

Insert

5myc-chkEn2(5E)-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Expression

Mutation

chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

Promoter

Zf-Ubi

Availability

Academic Institutions and Nonprofits only

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pTol2-nbt300 2n-KalNFB-3xmir1T-syt2a-10U2n-mCardinal-P2A-mClover3

Plasmid

#196117

Purpose

zebrafish expression

Depositor

Reinhard Köster

Insert

nbt300 2n-KalNFB-3xmir1T-syt2a-HO1-10U2n-mCardinal-P2A-mClover3

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTol2-F-actin-microtubule plus ends reporters

Plasmid

#196134

Purpose

zebrafish expression

Depositor

Reinhard Köster

Insert

F-actin-microtubule plus ends reporters

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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ZE46 DeltaPGB Dvl2 pCS2P+

Plasmid

#17087

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

Dvl-2 (dvl2 Zebrafish)

Use

Zebrafish expression

Tags

Expression

Mutation

DeltaPGB (deletes amino acids 231-252)

Promoter

Availability

Academic Institutions and Nonprofits only
pISceI Dusp6:memGFP

Plasmid

#32668

Depositor

Michael Tsang

Article

Wang et al Development. 2011 Jan;138(1):45-54. Epub

Insert

Dusp6 promoter (dusp6 Zebrafish)

Use

Zebrafish transgenesis

Tags

EGFP

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

Plasmid

#74010

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5xUAS
Cas9
Cre
U6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCRII HSPB8

Plasmid

#61035

Purpose

Probe for HSPB8 for in situ hybridization in zebrafish

Depositor

Lara Hutson

Article

Marvin et al Dev Dyn. 2008 Feb;237(2):454-63.

Insert

HSPB8 (hspb8 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pCS2-epNTR

Plasmid

#62213

Purpose

epNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2

Depositor

Harold Burgess

Article

Tabor et al J Neurophysiol. 2014 Aug 15;112(4):834-4

Insert

epNTR

Use

Mrna synthesis

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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mhc2dab:GFP-LT

Plasmid

#68996

Purpose

for making transgenic fish

Depositor

David Traver

Article

Wittamer et al Blood. 2011 Jun 30;117(26):7126-35. doi:

Insert

mhc2dab (mhc2dab Zebrafish)

Use

Zebrafish expression

Tags

GFP-LT

Expression

Mutation

Promoter

mhc2dab

Availability

Academic Institutions and Nonprofits only

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pAc-His-zfKitlgb

Plasmid

#140293

Purpose

Express soluble His-tagged zebrafish Kitlgb in insect cells

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlgb (kitlgb Zebrafish)

Use

Tags

6x His

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSws1-nfsB-CFP

Plasmid

#86375

Purpose

for zebrafish transgenesis, expression of nfsB-CFP, also expresses EGFP

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

nitroreductase (nfsB bacteria)

Use

Zebrafish

Tags

ECFP

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCS2-sod1

Plasmid

#107594

Purpose

Contains zebrafish sod1 gene in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

sod1 (sod1 Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT3TS-Hypa-nCas9n-A69

Plasmid

#125645

Purpose

micro-injection of poly-adenylated Hypa-nCas9n RNA

Depositor

Jason Berman

Article

Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

Insert

Hypa-nCas9n

Use

Rna in vitro transcription vector

Tags

Expression

Mutation

N692A, M694A, Q695A, H698A from wild-type Cas9

Promoter

T3

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnma1a

Plasmid

#164951

Purpose

Zebrafish kcnma1a gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnma1a (kcnma1a Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnn3

Plasmid

#164959

Purpose

Zebrafish kcnn3 gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnn3 (kcnn3 Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnn4

Plasmid

#164960

Purpose

Zebrafish kcnn4 gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnn4 (kcnn4 Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnt2a

Plasmid

#164963

Purpose

Zebrafish kcnt2a gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnt2a (kcnt2 Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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