-
Plasmid#233358PurposeCMV driven expression of the inactive PKC activity recorder negative control with T/A mutation Kinprola_PKC_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKC_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_on-mEGFP
Plasmid#233355PurposeCMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_on-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_AMPK_T/A-mEGFP
Plasmid#233362PurposeCMV driven expression of the inactive AMPK activity recorder negative control with T/A mutation Kinprola_AMPK_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_AMPK_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_AMPK-mEGFP
Plasmid#233361PurposeCMV driven expression of the AMPK activity recorder Kinprola_AMPK in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_AMPK-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKC-mEGFP
Plasmid#233357PurposeCMV driven expression of the PKC activity recorder Kinprola_PKC in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKC-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro_CMV_NES-Kinprola_PKA-mEGFP
Plasmid#233369PurposeCMV driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for neuronal expression through lentivirus transductionDepositorInsertNES-Kinprola_PKA-mEGFP
UseLentiviralTagsNES and mEGFPExpressionMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro_CMV_NES-Kinprola_on-mEGFP
Plasmid#233370PurposeCMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through lentivirus transductionDepositorInsertNES-Kinprola_on-mEGFP
UseLentiviralTagsNES and mEGFPExpressionMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-PspCas13b-NES-mCherry
Plasmid#233608PurposeExpression of PspCas13b in mammalian cellsDepositorInsertPspCas13b-NES-mCherry
UseCRISPRTags3xFlag and mCherryExpressionMutationPromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCI5-CasRx-NES-HA (pKW387)
Plasmid#229048PurposeMammalian expression of CasRx-NES-HADepositorInsertCasRx (RfxCas13d)
UseCRISPRTagsNES-HAExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-Eevee-ROCK-NES
Plasmid#209918PurposeA FRET biosensor for ROCK activityDepositorInsertEevee-ROCK-NES
UseLentiviralTagsECFP and YPetExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT309 RapInducible1-ActivatorCloneSite-Citrine
Plasmid#187321PurposeRapamycin inducible genetic circuit with cloning site for activator domainsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmax-PUFc_NES-ADARdd(E488Q)
Plasmid#196837PurposeExpress PUFc_NES-ADARdd(E488Q)DepositorInsertPUFc_NES-ADARdd(E488Q)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmax-MCP_NES-ADARdd(E488Q)
Plasmid#196840PurposeExpress MCP_NES-ADARdd(E488Q)DepositorInsertMCP_NES-ADARdd(E488Q)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_hSyn_NES-Caprola_05-mEGFP_WRPE-SV40
Plasmid#194689PurposehSyn1 driven expression of the calcium recorder Caprola_05 fused to mEGFP for neuronal expression through AAV transductionDepositorInsertCaprola_05-mEGFP
UseAAVTagsmEGFPExpressionMutationPromoterhSyn1Available sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (AG4TGGA)2
Plasmid#182488PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence (AG4TGGA)2 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free deadFPPS-NES
Plasmid#182492PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.DepositorInsertsFPPS
deadFPPS-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
ND6-L1397-N_UGI-NES_right
Plasmid#187846Purposeexpresses the right-side half ND6-UGI-NES in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–NES–ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
ND6-L1397-N_UGI-NES_left
Plasmid#187845Purposeexpresses the left-side half ND6-UGI-NES in mammalian cellsDepositorInsertSOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–hivNES–SOD2 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKD6-V5-APEX2-NES
Plasmid#178700PurposeAAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of V5-APEX2-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)DepositorInsertV5-APEX2-NES
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS2-V5-APEX2-NES
Plasmid#178702PurposeAAV vector for Cre-dependent transgene expression of V5-APEX2-NES in neurons under the control of the hSyn1 promoter (Kugler et al 2003)DepositorInsertV5-APEX2-NES
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only