Addgene

We narrowed to 23,179 results for: nes

Showing: 1 - 20 of 23179 results

Results per page:

Sort results by:

3NES-CS(G)-tTA-CS(G)-3NES

Plasmid

#137836

Purpose

Soluble tTA transcription factor flanked by N and C terminal TEV protease G cleavage sequences and 3 nuclear export sequences

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

3NES-CD(G)-tTA-CS(G)-3NES

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pECE M2-SH3PXD2A 8 serines mutated to alanines

Plasmid

#69816

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pECE M2-SH3PXD2A 6 serines mutated to alanines

Plasmid

#69815

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p23-NES-LwaCas13a-msfGFP-NES-Flag

Plasmid

#165070

Purpose

overexpression of LwaCas13a in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

LwaCas13a

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p23-NES-RfxCas13d-msfGFP-NES-Flag

Plasmid

#165076

Purpose

overexpression of RfxCas13d in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

RfxCas13d

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p23-NES-EsCas13d-msfGFP-NES-Flag

Plasmid

#165074

Purpose

overexpression of EsCas13d in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

EsCas13d

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p23-NES-AdmCas13d-msfGFP-NES-Flag

Plasmid

#165075

Purpose

overexpression of AdmCas13d in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

AdmCas13d

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p23-NES-PspCas13b-msfGFP-NES-Flag

Plasmid

#165071

Purpose

overexpression of PspCas13b in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

PspCas13b

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p23-NES-PguCas13b-msfGFP-NES-Flag

Plasmid

#165072

Purpose

overexpression of PguCas13b in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

PguCas13b

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p23-NES-RanCas13b-msfGFP-NES-Flag

Plasmid

#165073

Purpose

overexpression of RanCas13b in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

RanCas13b

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pNES-SET-EMD-EGFP (C-NES-SET-EMD)

Plasmid

#217770

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

SET (SET Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

EMD domain (aa 70-226) plus nuclear export sequen…

Promoter

CMV

Available since

June 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pNES-PRKCZ-C20-EGFP (C-NES-PRKCZ-C20)

Plasmid

#217763

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

NES (ALQKKLEELELDEAPVAT) plus C20 domain (aa 405-…

Promoter

CMV

Available since

June 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
NES-Halo

Plasmid

#200201

Purpose

Expression of 3X HIV NES

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

3X HIV Rev NES-Halo

Use

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GA-NES

Plasmid

#61018

Purpose

ddGFP A-NES

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP A

Use

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
HyPer7.2DAAO-NES

Plasmid

#168301

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the cytoplasm

Depositor

Thomas Michel

Article

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-NES

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GB-NES

Plasmid

#61017

Purpose

ddGFP B-NES

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP B

Use

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
RA-NES

Plasmid

#61019

Purpose

ddRFP A-NES

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddRFP A

Use

Tags

HindIII site and NES sequence MNLVDLQKKLEELELDEQQ

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pmTurquoise2-NES

Plasmid

#36206

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

NES

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pSOMA-NES

Plasmid

#118939

Purpose

Used to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

Depositor

Patrick Krysan

Article

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TND-NES

Plasmid

#210726

Purpose

NIR light-activated intein-based gene expression system (N-part)

Depositor

Vladislav Verkhusha

Article

Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

Insert

TND-NES

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge