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Plasmid#108895PurposeMultisite gateway vector for 5' tagging with APEX2 (includes cytoplasmic GFP reporter)DepositorInsertEGFP-P2A-APEX2
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceMay 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAS-Cre
Plasmid#50797PurposeExpression of Cre recombinase under the control of the UAS-promoterDepositorInsertCre recombinase
UseMulti-speciesTagsExpressionMutationPromoterUAS-hsp70Available sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYTSG
Plasmid#159419PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiaeDepositorInsertthermostable GFP (TGP)
UseTagspolyhistidine (poly-His)ExpressionYeastMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3CA_MBP
Plasmid#132700Purposeexpresses Avi-tagged MBP in E.coli, to use as positive control in ELISADepositorInsertMaltose binding Protein
UseTagsAvi and HisExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFTSG
Plasmid#162389PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cellDepositorInsertthermostable GFP (TGP)
UseTagsHisExpressionInsectMutationPromoterAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-7D12Gly42TAG
Plasmid#125265PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gly42 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsert7D12 Gly42 nanobody
UseTagsHis-tagExpressionBacterialMutationPromotertrc promoterAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NbALFA
Plasmid#159987PurposeFor the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmCherry-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mCherry and NbALFAPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-NbALFA
Plasmid#159986PurposeFor the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmEGFP-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mEGFP and NbALFAPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEHlyA5
Plasmid#168298PurposeSecretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyADepositorInsertN‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
UseTagsE-tag, HA-tag, His-tag, Prescission site, and VHH…ExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSC130
Plasmid#216364PurposexhNup98-Nb3i Nanobody productionDepositorInsertxhNup98-Nb3i
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTP443
Plasmid#216361PurposexNup98-Nb1t (+1Cys) Nanobody productionDepositorInsertxNup98-Nb1t (+1Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry::Nrv1::vhhGFP4
Plasmid#163926PurposeExpression of GrabFP-B-intracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::Vkg::mCherry
Plasmid#163929PurposeExpression of GrabFP-ECM under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 nanobody with Viking (Collagen IV) protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationG1767DPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDG04439
Plasmid#216365PurposexhNup133-Nb2t (+2Cys) Nanobody productionDepositorInsertxhNup133-Nb2t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTP489
Plasmid#216366PurposexNup155-Nb1t (+3Cys) Nanobody productionDepositorInsertxNup155-Nb1t (+3Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC263
Plasmid#216367PurposexhNup155-Nb2i Nanobody productionDepositorInsertxhNup155-Nb2i
UseAffinity Reagent/ AntibodyTags14xHis-Avi-SUMOEu1ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC236
Plasmid#216368PurposexhNup155-Nb2i (+2Cys) Nanobody productionDepositorInsertxhNup155-Nb2i (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC100
Plasmid#216369PurposexhNup155-Nb2i Nanobody productionDepositorInsertxhNup155-Nb2i
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC99
Plasmid#216370PurposexhNup155-Nb3i Nanobody productionDepositorInsertxhNup155-Nb3i
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC261
Plasmid#216371PurposexhNup155-Nb3i Nanobody productionDepositorInsertxhNup155-Nb3i
UseAffinity Reagent/ AntibodyTags14xHis-Avi-SUMOEu1ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only