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Plasmid#26181DepositorInsertC terminal VSV tag
UseSynthetic Biology; 2ab assembly vectorTagsExpressionMutationPromoterAvailable sinceJune 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP
Plasmid#171090PurposeCRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.DepositorInsertsSth1 dCas9
Chloramphenicol acetyltransferase
TagBFP
UseCRISPR; Toxoplasma gondii expressionTags3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…ExpressionMutationD9A, H599APromoterTUB1 (Toxoplasma gondii), transcriptionally linke…Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AlcR (GB1550)
Plasmid#160585PurposeEthanol dependent transcrition factorDepositorInsertAlcR
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVtight Neo DEST (w770-1)
Plasmid#26432DepositorTypeEmpty backboneUseLentiviral; Tet-on advanced destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMSCVhyg-DEST
Plasmid#119747PurposeAllows Gateway cloning of gene of interest into retroviral pMSCV vector (with Hygromycin resistance)DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOT2-poly-cis
Plasmid#141177PurposeTo clone any gene or STTM sequence or artificial miRNA under CaMV 35S promoterDepositorTypeEmpty backboneUseRNAi and Synthetic BiologyTagsExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 GFP-NT (GB2236)
Plasmid#160590PurposeGFP for N-terminal fusionsDepositorInsertGFP-NT
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.3_M1-gateway-HA
Plasmid#128439Purposelentiviral destination vector with C-terminal HA tagDepositorInsertC-terminal HA tag
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 CBS4 (GB2718)
Plasmid#160601PurposeCopper dependent CUP2 binding DNA sequenceDepositorInsertCBS4
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG416GALL-ccdB-6stop
Plasmid#203511PurposeGateway-compatible destination vector for yeast expression with GALL promoter/6stop mutation/URA3 markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-ccdB-6stop
Plasmid#203512PurposeGateway-compatible destination vector for yeast expression with GAL10 promoter/6stop mutation/URA3 markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-PB-R4R3
Plasmid#139518PurposeProvides two PiggyBac transposon sequences between gene cassettes in the destination vectorDepositorTypeEmpty backboneUsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.4_M1-gateway-V5
Plasmid#128440Purposelentiviral destination vector with C-terminal V5 tagDepositorInsertC-terminal V5 tag
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 GFP-CT (GB2238)
Plasmid#160592PurposeGFP for C-terminal fusions.DepositorInsertGFP-CT
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM-PAT-35S-GW-Luc
Plasmid#80678Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterPro35SAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
plix403-V5-N-mEYFP
Plasmid#223955PurposeGateway cloning of genes of interest with an N-terminal V5-mEYFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseLentiviralTagsV5-mEYFPExpressionMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
plix403-V5-N-mEGFP
Plasmid#223957PurposeGateway cloning of genes of interest with an N-terminal V5-mEGFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseLentiviralTagsV5-mEGFPExpressionMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
plix403-V5-N-mECFP
Plasmid#223954PurposeGateway cloning of genes of interest with an N-terminal V5-mECFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseLentiviralTagsV5-mECFPExpressionMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
plix403-V5-N-mCherry
Plasmid#223956PurposeGateway cloning of genes of interest with an N-terminal V5-mCherry fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseLentiviralTagsV5-mCherryExpressionMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGENT R2 GW R1 RUBY
Plasmid#225988PurposeTo express RUBY transgene under the control of desired promoter (Note: R2 Gateway R1)DepositorTypeEmpty backboneUseTagsRUBYExpressionPlantMutationPromoterAvailable sinceOct. 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits