Skip to main content
Addgene
Showing: 421 - 440 of 548 results

  1. pEG608

    Plasmid
    #40194
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (RNAi resistant) (mex-5 Nematode)
    Use
    Tags
    3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…
    Expression
    Worm
    Mutation
    Exon 2 recoded to be RNAi-resistant
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 19, 2012
    Availability
    Academic Institutions and Nonprofits only

  5. SpyTag-sfGFP

    Plasmid
    #184226
    Purpose
    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpyTag-sfGFP
    Use
    Tags
    His6, SpyTag, and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184226-plasmid-map-sequence-id-361605

    Enlarge

  11. pMSP1D1

    Plasmid
    #20061
    Purpose
    Bacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477
    Insert
    MSP1D1
    Use
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    deletion mutant (1-11) of MSP1; His-tag on N-term…
    Promoter
    Available since
    March 16, 2009
    Availability
    Academic Institutions and Nonprofits only
    20061-plasmid-map-sequence-id-354445

    Enlarge

  14. Fireworks RFP[PTC-] (AVA2515)

    Plasmid
    #85443
    Purpose
    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.
    Depositor
    Joan Steitz
    Article
    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:
    Insert
    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-HygromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85443-plasmid-map-sequence-id-171526

    Enlarge

  15. RP1B-PP1alpha

    Plasmid
    #51768
    Purpose
    Expresses PP1 alpha residues 7-330
    Depositor
    Wolfgang Peti
    Article
    Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.
    Insert
    pp1 alpha
    Use
    Tags
    His, TEV protease site, and Thio-6
    Expression
    Bacterial
    Mutation
    Contains PP1 alpha residues 7-330
    Promoter
    T7-lac
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pCL1032

    Plasmid
    #217962
    Purpose
    Mammalian expression of NASP ('s' isofrom) fused at its N-terminus to eGFP with a TEV protease site separating the two. Puromycin resistance is expressed from a downstream IRES.
    Depositor
    Andrew Bowman
    Article
    Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1
    Insert
    NASP (NASP Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217962-plasmid-map-sequence-id-430216

    Enlarge

  19. pEG584

    Plasmid
    #40191
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (RNAi resistant) (mex-5 Nematode)
    Use
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    Exon 2 recoded to be RNAi-resistant
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 5, 2012
    Availability
    Academic Institutions and Nonprofits only

  20. pEG587

    Plasmid
    #40196
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (S458A) (RNAi resistant) (mex-5 Nematode)
    Use
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    S458A; exon 2 recoded to be RNAi-resistant
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 19, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 421 - 440 of 548 results