Showing: 421 - 440 of 2818 results
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Plasmid#123613PurposeCAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).DepositorInsertBE3(E63Q)-P2A-EGFP
UseTagsExpressionMammalianMutationE63Q in rAPOBEC1, D10A in SpCas9PromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
SECURE BE3(R33A)-P2A-EGFP (pJUL1085)
Plasmid#123614PurposeCAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).DepositorInsertBE3(R33A)-P2A-EGFP
UseTagsExpressionMammalianMutationR33A in rAPOBEC1, D10A in SpCas9PromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)
Plasmid#123615PurposeCAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).DepositorInsertBE3(R33A/K34A)-P2A-EGFP
UseTagsExpressionMammalianMutationR33A/K34A in rAPOBEC1, D10A in SpCas9PromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
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pORFE1052
Plasmid#112078PurposeOverexpression of the AtCAS9 nickase in plant under 2x35S promoterDepositorInsertAtCAS9 D10A
UseCRISPRTagsExpressionPlantMutationPlant codon optimized nCas9 D10A S. pyogenesPromoter2x35SAvailabilityAcademic Institutions and Nonprofits only -
pX335 Mouse 5' Srcap gRNA A
Plasmid#127904PurposeCas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap geneDepositorInsertSrcap gRNA
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pX335 Mouse 5' Srcap gRNA B
Plasmid#127905PurposeCas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap geneDepositorInsertSrcap gRNA
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDCC5
Plasmid#59984PurposeBi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassetteDepositorInsertCas9-D10A
UseCRISPRTagsHAExpressionInsectMutationD10APromoterhsp70BbAvailabilityAcademic Institutions and Nonprofits only -
Human ICAM-2
Plasmid#8634DepositorInsertICAM-2 (ICAM2 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ265L2
Plasmid#174000PurposeGateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editingDepositorInsertUNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationIn rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ265N2
Plasmid#174001PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editingDepositorInsertrAPOBEC1-zCas9(D10A)-SpRY-UNG
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationIn zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ265O2
Plasmid#174002PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editingDepositorInsertrAPOBEC1-zCas9(D10A)-SpRY-rXRCC1
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationIn zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …PromoterAvailabilityAcademic Institutions and Nonprofits only -
AIO-Puro
Plasmid#74630PurposeAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editingDepositorTypeEmpty backboneUseCRISPRTagsPuromycin resistant markerExpressionMammalianMutationPromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pLenti-Arch-EEN
Plasmid#45189PurposeArch voltage indicator with double mutations D95N-D106E (Arch-EEN), with faster kinetics and greater fluorescence dynamic range than Arch-D95NDepositorInsertenhanced Archaerhodopsin-3
UseLentiviralTagseYFPExpressionMammalianMutationD95N, D106EPromoterCamKIIaAvailabilityAcademic Institutions and Nonprofits only -
pCR1003
Plasmid#71821PurposeExpress Streptococcus pyogenes dCas9 (D10A, H840A)DepositorInsertT7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCR1054
Plasmid#71822PurposeExpress Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCBEclos-cbei2385-g2-opt
Plasmid#118215PurposeCRISPR-mediated base editing in ClostridiumDepositorInsertApobec1, Casp nickase, UG1-sgRNA
UseTagsExpressionBacterialMutationPromoterPtmAvailabilityAcademic Institutions and Nonprofits only -
pRC02
Plasmid#114681PurposesfGFP.Strand10-HaloenzymeDepositorInsertsfGFP.Strand10-Haloenzyme
UseTagsHis10ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pH-ABE8e
Plasmid#200774PurposeFor plant adenine base editing in rice plants or monocotyledons protoplastsDepositorInsertnCas9(D10A)-TadA8e
UseCRISPRTagsExpressionPlantMutationD10A for Cas9; W23R, H36L, P48A, R51L, L84F, A106…Promotermaize Ubiquitin-1, OsU3AvailabilityAcademic Institutions and Nonprofits only -
pH-ABE8e-spG
Plasmid#200775PurposeFor plant adenine base editing in rice plants or monocotyledons protoplastsDepositorInsertnCas9-spG(D10A)-TadA8e
UseCRISPRTagsExpressionPlantMutationD10A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…Promotermaize Ubiquitin-1, OsU3AvailabilityAcademic Institutions and Nonprofits only
Showing: 421 - 440 of 2818 results