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Showing: 4361 - 4380 of 4600 results

  2. pLuc-Optop38i-dsm

    Plasmid
    #89748
    Purpose
    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F
    Depositor
    Michael Courtney
    Publication
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    Optop38i dark-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89748-plasmid-map-sequence-id-192493

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  4. pET28a HaloTag-TEVc-6xtrTUBE

    Plasmid
    #254153
    Purpose
    Bacterial expression of 6xtrTUBE with an N-terminal HaloTag followed by a TEV protease cleavage site
    Depositor
    Rahul Samant
    Publication
    Johnston et al J. Proteome Res. 2026
    Insert
    6xtrTUBE (UBQLN1 Human, Synthetic)
    Tags
    HaloTag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    All Arg residues in the UBA domain are mutated to…
    Promoter
    T7
    Available since
    Aug. 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    254153-plasmid-map-sequence-id-511309

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  7. pET21a(+)-Thio6-His6-TEV-TARDBP(Δ1-273, W385tag)

    Plasmid
    #260639
    Purpose
    Expresses TDP-43(Δ1-273, W385tag) for Amber codon suppression in bacterial strains
    Depositor
    Jennifer Lee
    Publication
    Watson et al J Am Chem Soc. 2026 Jun 17;148(23):23494
    Insert
    Transactive response DNA binding protein 43 (TARDBP Human)
    Tags
    thio6 expression sequence, hexahistidine, TEV pro…
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-273, mutated Tryptophan 385…
    Promoter
    T7
    Available since
    Sept. 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    260639-plasmid-map-sequence-id-521733

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  8. pET28a 6xHis-HaloTag-TEVc-4xtrTUBE

    Plasmid
    #254154
    Purpose
    Bacterial expression of 4xtrTUBE with an N-terminal 6xHis and HaloTag followed by a TEV protease cleavage site
    Depositor
    Rahul Samant
    Publication
    Johnston et al J. Proteome Res. 2026
    Insert
    4xtrTUBE (UBQLN1 Human, Synthetic)
    Tags
    HaloTag, His6, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    All Arg residues in the UBA domain are mutated to…
    Promoter
    T7
    Available since
    Aug. 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    254154-plasmid-map-sequence-id-511316

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  9. pET28a 6xHis-HaloTag-TEVc-6xtrTUBE

    Plasmid
    #254156
    Purpose
    Bacterial expression of 6xtrTUBE with an N-terminal 6xHis and HaloTag followed by a TEV protease cleavage site
    Depositor
    Rahul Samant
    Publication
    Johnston et al J. Proteome Res. 2026
    Insert
    6xtrTUBE (UBQLN1 Human, Synthetic)
    Tags
    6xHis, HaloTag, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    All Arg residues in the UBA domain are mutated to…
    Promoter
    T7
    Available since
    Aug. 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    254156-plasmid-map-sequence-id-516711

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  10. pcDNA6-BKαM3αM4-V5-His

    Plasmid
    #255912
    Purpose
    BKαM dual-repeat expressing plasmid in which αM3 (residues 44–649) is preceded by an N-terminal sequence (MGAAAA) containing a NotI site and linked to αM4 (residues 43–649) via a flexible linker.
    Depositor
    Jiusheng Yan
    Publication
    Chen et al Elife. 2026 Mar 5;14:RP107681. doi: 10.7
    Insert
    KCNMA1 (partial) (KCNMA1 Human)
    Tags
    V5-6xHis
    Expression
    Mammalian
    Mutation
    contains αM3 (residues 44–649) preceded by an N-t…
    Available since
    June 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    255912-plasmid-map-sequence-id-513608

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  11. pcDNA6-myc-BKαΔM-GFP-V5-His

    Plasmid
    #255910
    Purpose
    Complementary plasmid for co-expression with BKαΔM module to form functional BK channels. BKα residues 94–651 are replaced by a flexible peptide linker and GFP is tagged at C-terminus.
    Depositor
    Jiusheng Yan
    Publication
    Chen et al Elife. 2026 Mar 5;14:RP107681. doi: 10.7
    Insert
    KCNMA1 (partial) (KCNMA1 Human)
    Tags
    EGFP-V5-6xHis and Myc
    Expression
    Mammalian
    Mutation
    residues 94–651 are deleted and replaced by a fle…
    Available since
    June 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    255910-plasmid-map-sequence-id-512404

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  12. pSIN-WP-mPGK-GDNF-IRES-tTA-KRAB

    Plasmid
    #225488
    Purpose
    Tet-on lentiviral vector for controlled expression of GDNF
    Depositor
    Robert Gross
    Publication
    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d
    Insert
    Tetracycline-controlled transactivator fused with Krüppel-associated box (KRAB) domain following an internal ribosome entry site
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Mouse phosphoglycerate kinase 1
    Available since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    225488-plasmid-map-sequence-id-444525

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  14. pGGA-FT guide RNA1_7

    Plasmid
    #246797
    Purpose
    A GreenGate entry vector containing a guide RNA expression cassette targeting the AtFT gene with 7 different gRNAs
    Depositor
    Danhua Jiang
    Insert
    AtU6-1 promoter-FT guide RNA1-AtU6-1 promoter-FT guide RNA3- (FT Mustard Weed, Synthetic)
    Use
    CRISPR; Greengate cloning entry vector
    Expression
    Plant
    Available since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    246797-plasmid-map-sequence-id-495594

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  16. pET28a 6xHis-HaloTag-TEVc-4xtrTUBEmut

    Plasmid
    #254155
    Purpose
    Bacterial expression of binding-deficient 4xtrTUBEmut with an N-terminal 6xHis and HaloTag followed by a TEV protease cleavage site
    Depositor
    Rahul Samant
    Publication
    Johnston et al J. Proteome Res. 2026
    Insert
    4xtrTUBEmut (UBQLN1 Human, Synthetic)
    Tags
    HaloTag, His6, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    All Arg residues in the UBA domain are mutated to…
    Promoter
    T7
    Available since
    Aug. 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    254155-plasmid-map-sequence-id-511310

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  17. pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin

    Plasmid
    #237480
    Purpose
    Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding.
    Depositor
    Dirk Daelemans
    Publication
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate)
    Use
    Lentiviral
    Tags
    IRES-mNeonGreen-NES/PKI and P2A-Blasticidin
    Expression
    Mammalian
    Mutation
    S484D + S813I + deleted amino acids 68-76 + 676-6…
    Promoter
    CMV
    Available since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    237480-plasmid-map-sequence-id-477747

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  18. LifeAct-mNeonGreen -IRES- Talin head-SpyTag003

    Plasmid
    #210024
    Purpose
    Expression of LifeAct-mNeonGreen and Talin head(1-433)-SpyTag003 in mammalian cells
    Depositor
    Vesa Hytönen
    Publication
    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459
    Insert
    LifeAct-mNeonGreen co-expressed with Talin head(1-433)-SpyTag003
    Expression
    Mammalian
    Mutation
    Encephalomyocarditis virus internal ribosome entr…
    Promoter
    CMV
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210024-plasmid-map-sequence-id-417730

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  19. pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)

    Plasmid
    #192784
    Purpose
    Expression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Publication
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and mXFPm
    Expression
    Mammalian
    Mutation
    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192784-plasmid-map-sequence-id-379372

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  20. pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

    Plasmid
    #131256
    Purpose
    Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)
    Depositor
    Roger Woodgate
    Publication
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Contains point mutations that convert USP7 aspart…
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131256-plasmid-map-sequence-id-267711

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Showing: 4361 - 4380 of 4600 results