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Plasmid#22994DepositorInsertSmad1 (SMAD1 Human)
UseTagsFLAGExpressionMammalianMutationS214A, S206A, S195A, S187A, S463E, S465EPromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pMTP140 (pBAD322G_TniQ-Cascade(Tn6900))
Plasmid#164262PurposeVector expressing the TniQ, Cas8/5, Cas7, and Cas6 proteins from the Tn6900 element with an arabinose promoter.DepositorInsertCodon-optimized tniQ-cas8/5-cas7-cas6 operon from Tn6900 from A. salmonicida S44 pS44-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-mPlum
Plasmid#158062PurposeDonor plasmid for insertion of mPlum and the constitutive acpP promoter into the S. Typhimurium chromosome. mPlum is in the forward orientation relative to the kanamycin resistance selectable markerDepositorInsertmonomeric plum fluoresecent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteracpPAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NES-MBP-cATOM(Ras)
Plasmid#209711PurposeMammalian expression of c-ATOM biosensor for HRas with nuclear export signalDepositorInsertNES-MBP-cATOM(Ras)
UseTagsNES-MBPExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFosill-3
Plasmid#89697Purposeto make Fosmids that can be converted into Illumina sequencable librariesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMP4518
Plasmid#107778PurposeExpression of EYFPDepositorInsertpLacZ-EYFP in pBBR1-mcs5
UseTagspLacZ-EYFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGM55285
Plasmid#153212PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD63-(TEV)-tTA
Plasmid#162597Purposehuman CD63-TEV cleavage site-tTADepositorInserthuman CD63 (CD63 Human)
UseTagsTEV protease cleavage site and tTAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM38869
Plasmid#153220PurposeLevel 0 Arabidopsis U6-promoter vectorDepositorInsertArabidopsis U6 snRNA promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AaCas12b-2AeGFP
Plasmid#121945PurposeMammalian expression, Genome editingDepositorInsertAaCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICSL90003
Plasmid#68262PurposeLevel 0 Golden Gate Part: Promoter U6 (Triticum aestivum)DepositorInsertPromoter: U6 (Triticum aestivum)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
mc2 mCherry
Plasmid#206219PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
PH-PLCD1_mScarlet-I_IRES_sYFP2_PH_N1
Plasmid#110623PurposeIt expresses separate mScarletI pHdomain and SYFP2 pHdomain in a 3:1 ratio.DepositorInsertPH mScarlet-I- IRES- sYFP2 PH
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaNTD
Plasmid#157869Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the N terminal domainDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationdeltaNTDPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Gh
Plasmid#61648PurposeGateway vector for generating green fluorescent protein fusions in fungiDepositorInsertenhanced green fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-Rab7A Q86L
Plasmid#28050DepositorInsertRab7A (RAB7A Human)
UseTags2xHA and 3xFlagExpressionMammalianMutationQ86LPromoterAvailable sinceJune 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLC242-GFP-Sestrin2
Plasmid#100519PurposeExpresses GFP tagged Sestrin2 in mammalian cells to make lentivirusDepositorInsertSESN2 (SESN2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-scrCdh2.1 GFP
Plasmid#209100PurposeGFP expressing scramble of shRNA targeting Cdh2DepositorInsertscrCdh2.1
UseTagsExpressionMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TR-Csy4-H29A
Plasmid#80602PurposeExpresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertCsy4-H29A
UseAAVTagsExpressionMammalianMutationHis29 mutated to AlaPromoterCBAAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only