Skip to main content
Addgene
Showing: 4321 - 4340 of 23065 results

  3. pET28a-FKBP-STING(pLxIS)

    Plasmid
    #221333
    Purpose
    Overexpression of STING CTT in bacteria
    Depositor
    Jonathan Kagan
    Insert
    STING CTT (Sting1 Mouse)
    Use
    Tags
    6xHis-4xFKBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221333-plasmid-map-sequence-id-438971

    Enlarge

  6. pET23b-2E2-HA-mEGFP-6xHis

    Plasmid
    #129594
    Purpose
    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    Use
    Tags
    6xHis-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    129594-plasmid-map-sequence-id-261157

    Enlarge

  7. pET-PE2-His (IK1822)

    Plasmid
    #170103
    Purpose
    T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS
    Depositor
    Keith Joung
    Article
    Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103
    Insert
    bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
    Use
    CRISPR
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Bacteria codon-optimized nSpCas9 (H840A) & M-…
    Promoter
    T7
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    170103-plasmid-map-sequence-id-332603

    Enlarge

  8. pET-22b DT 51E/148K

    Plasmid
    #11081
    Depositor
    John Collier
    Insert
    DT
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    51E/148K, catalytically inactive
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    11081-plasmid-map-sequence-id-306912

    Enlarge

  10. pET-His6-SUMO-MproC145A

    Plasmid
    #172653
    Purpose
    Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO
    Depositor
    Stephen Harrison
    Article
    MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25
    Insert
    SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHis Tag and SUMO
    Expression
    Bacterial
    Mutation
    Catalytic Cysteine 145 mutated to Alanine
    Promoter
    T7
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    172653-plasmid-map-sequence-id-370049

    Enlarge

  18. pET-MBP-NLS-Geo_st

    Plasmid
    #87703
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS
    Depositor
    Jennifer Doudna
    Insert
    GeoCas9
    Use
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87703-plasmid-map-sequence-id-176992

    Enlarge

  20. pET28-AviTag-DogTag-MBP

    Plasmid
    #171773
    Purpose
    Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogTag-MBP
    Use
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Peptide tag added to N-terminus of MBP via a GSGE…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171773-plasmid-map-sequence-id-338517

    Enlarge

Showing: 4321 - 4340 of 23065 results