Skip to main content
Addgene
Showing: 4301 - 4320 of 9139 results

  1. pJK697

    Plasmid
    #71646
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met15>Trp/Ser38>Thr mutant (TdPurE2-M15W,S38T)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes methionine-15 to tryptophan, serine-38 to…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71646-plasmid-map-sequence-id-131667

    Enlarge

  2. pJK657

    Plasmid
    #71647
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His40>Ala/Phe79>Trp mutant (TdPurE2-H40A,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-40 to alanine, phenylalanine-79…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71647-plasmid-map-sequence-id-131642

    Enlarge

  3. pJK658

    Plasmid
    #71648
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His40>Asn/Phe79>Trp mutant (TdPurE2-H40N,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-40 to asparagine, phenylalanine…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71648-plasmid-map-sequence-id-131644

    Enlarge

  4. pJK505

    Plasmid
    #71594
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe mutant (AaPurE1fww)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, additiona…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71594-plasmid-map-sequence-id-131669

    Enlarge

  5. pJK506

    Plasmid
    #71596
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp165>Phe mutant (AaPurE1wwf)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-165 to phenylalanine, addition…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71596-plasmid-map-sequence-id-131670

    Enlarge

  9. R777-E164 Hs.PIK3CA-nostop

    Plasmid
    #70448
    Purpose
    Gateway ORF clone of human PIK3CA [NM_006218.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3CA (PIK3CA Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70448-plasmid-map-sequence-id-125897

    Enlarge

  10. YWHAE

    Plasmid
    #39146
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    YWHAE (YWHAE Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. YWHAQ

    Plasmid
    #38931
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    YWHAQ (YWHAQ Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. R777-E036 Hs.DUSP2-nostop

    Plasmid
    #70320
    Purpose
    Gateway ORF clone of human DUSP2 [NM_004418.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    DUSP2 (DUSP2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70320-plasmid-map-sequence-id-125769

    Enlarge

  13. H2B-RFP in pENTR1A (w507-1)

    Plasmid
    #22525
    Depositor
    Eric Campeau
    Insert
    Human Histone H2B -Heteractis crispa red fluorescent protein (H2BC21 Human)
    Use
    Entry vector
    Tags
    HcRed
    Expression
    Mutation
    Promoter
    Available since
    Jan. 22, 2010
    Availability
    Academic Institutions and Nonprofits only
    22525-plasmid-map-sequence-id-10355

    Enlarge

  14. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Nathan Reyna
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    68374-plasmid-map-sequence-id-121945

    Enlarge

  15. R777-E297 Hs.RPS6KB1

    Plasmid
    #70581
    Purpose
    Gateway ORF clone of human RPS6KB1 [NM_003161.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RPS6KB1 (RPS6KB1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70581-plasmid-map-sequence-id-126030

    Enlarge

  16. pCAG-IRES2-p75NTR-GFP

    Plasmid
    #182947
    Purpose
    To express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-Terminal
    Depositor
    Pantelis Tsoulfas
    Insert
    p75NTR-mGFP
    Use
    Tags
    HA tag and mGFP
    Expression
    Mammalian
    Mutation
    The leader sequence is from preprotrypin
    Promoter
    CAG
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182947-plasmid-map-sequence-id-360205

    Enlarge

  18. pENTR/shp16 (w98-1)

    Plasmid
    #22258
    Depositor
    Eric Campeau
    Insert
    sh cyclin-dependent kinase inhibitor 2A (CDKN2A Human)
    Use
    RNAi; Entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2009
    Availability
    Academic Institutions and Nonprofits only
    22258-plasmid-map-sequence-id-10198

    Enlarge

  19. R777-E023 Hs.CDK4

    Plasmid
    #70307
    Purpose
    Gateway ORF clone of human CDK4 [NM_000075.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    CDK4 (CDK4 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70307-plasmid-map-sequence-id-125756

    Enlarge

  20. R777-E021 Hs.CCND3

    Plasmid
    #70305
    Purpose
    Gateway ORF clone of human CCND3 [NM_001760.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    CCND3 (CCND3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70305-plasmid-map-sequence-id-125754

    Enlarge

Showing: 4301 - 4320 of 9139 results