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Plasmid#130821PurposeExpresses LifeAct-iFAST in mammalian cells (actin labeling)DepositorInsertLifeAct-iFAST
UseTagsmyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-TA Bik-IRES-Puromycin
Plasmid#133026PurposeExpresses EGFP-tagged tail anchor sequence of BikDepositorInsertBcl-2-interacting killer (BIK Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 1-110PromoterHuman elongation factor 1 alphaAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pONL-C1_Rab11a
Plasmid#65707PurposeExpresses ONL-Rab11a in mammalian cellsDepositorInsertRab11a (Rab11a Mouse)
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-YNL-CP
Plasmid#65714PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)DepositorInsert7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-ONL-CP
Plasmid#65716PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)DepositorInsert7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1α1.1-SomArchon-GFP
Plasmid#153531PurposeAAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter.DepositorInsertSomArchon-GFP
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationPromoterEF1α1.1Available sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-p160(400-580)
Plasmid#45DepositorInsertp160 myb binding protein (Mybbp1a Mouse)
UseTagsmycExpressionMammalianMutationexpresses only aa400-580PromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-p160(200-400)
Plasmid#44DepositorInsertp160 myb binding protein (Mybbp1a Mouse)
UseTagsmycExpressionMammalianMutationExpresses only aa200-400PromoterAvailable sinceJuly 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAG498 MAP4-iFAST
Plasmid#130820PurposeExpresses MAP4-iFAST in mammalian cells (microtubule labeling)DepositorInsertMAP4-iFAST
UseTagsMyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pluc-miR148a-148aT
Plasmid#65053PurposeReporter plasmid to detect miR148/152 family expressionDepositorInsertEngineered target sites miR-148a-3p (MIR148A Human, Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-CNL-CP
Plasmid#65715PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)DepositorInsert7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
3389 pcDNA3 Bid p22
Plasmid#8807DepositorInsertBid p22 (Bid Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
DF-UAS-spa-GCaMP6f
Plasmid#67568PurposeZebrafish expression of a photoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6f
UseZebrafish transgenesis (see :a spinal opsin contr…TagsHIS-tagExpressionMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…PromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLS2965
Plasmid#122534PurposeExpression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a Golgi localized gene (aman-2) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_aman-2::CemOrange2 (aman-2 Synthetic, Nematode)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
GAL-p300-230-670
Plasmid#89099Purposemammalian expression of human p300 amino acids 230-670 with N-terminal GAL4 DNA binding domainDepositorInsertp300 (EP300 Human)
UseTagsGAL4 DBDExpressionMammalianMutationaa 230-670PromoterAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
GAL-p300-2-300
Plasmid#89098Purposemammalian expression of human p300 amino acids 2-300 with N-terminal GAL4 DNA binding domainDepositorInsertp300 (EP300 Human)
UseTagsGAL4 DBDExpressionMammalianMutationaa 2-300PromoterAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL
Plasmid#63886PurposeAn AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.DepositorInsertER-localized GCaMP3(wt)
UseAAVTagsExpressionMutationPromoterhuman SYN1Available sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_Lys322Arg
Plasmid#105862PurposeExpression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Lys322Arg (EU numbering).DepositorInsertIgG3 heavy chain (Ighg3 Mouse)
UseTagsExpressionMammalianMutationLys322Arg in constant region of IgG1 heavy chainPromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
C-His-GvpC-TEV
Plasmid#153296PurposeExpresses TEV-sensitive variant of Anabaena flos-aquae GvpC in E.coliDepositorInsertTEV-sensitive Gas vesicle protein C (his-tagged)
UseTagsHis-tagExpressionBacterialMutation1) 21 bp TEV recognition and cleavage site flanke…PromoterT7Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
N-His-GvpC-ssrA
Plasmid#153297PurposeExpresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coliDepositorInsertGas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
UseTagsHis-tag and ssrA tagExpressionBacterialMutation1) N-terminal 6xHis tag flanked by 1x glycine res…PromoterT7Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only