Addgene

We narrowed to 6,210 results for: mag

Showing: 4301 - 4320 of 6210 results

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pCNL-C1_fibrillarin

Plasmid

#65709

Purpose

Expresses CNL-fibrillarin in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

fibrillarin (FBL Human)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
AAV-FLEX-tmeGFP-IRES-nls-mRuby2

Plasmid

#63060

Purpose

Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

nls-mRuby2

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-Fbxo7(ΔF-box)-IRES-GFP

Plasmid

#171787

Purpose

retrovirus production

Depositor

Heike Laman

Article

Lomonosov et al PLoS One. 2011;6(6):e21165. doi: 10.1371

Insert

Fbxo7 isoform 1 (FBXO7 Human)

Use

Retroviral

Tags

Expression

Mutation

deletion of aa335-367

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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mTFP1-LaminB1-7

Plasmid

#55492

Purpose

Localization: Nuclear Envelope, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

LaminB1 (LMNB1 Human)

Use

Tags

mTFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-TOMM20-N-10

Plasmid

#55511

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

TOMM20 (TOMM20 Human)

Use

Tags

mTFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pICE-EGFP-FLAG-Ku70siR-Mut6E

Plasmid

#46962

Purpose

For constitutive or doxycycline-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

Ku70 (XRCC6 Human)

Use

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Mut6E corresponding to K282E K287E T300E K331E K3…

Promoter

CMV Tet

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pSOMA-T679A-NES

Plasmid

#118940

Purpose

Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

Depositor

Patrick Krysan

Article

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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mCardinal-Rab4a-7

Plasmid

#56174

Purpose

Localization: Ras GTPase, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Rab4a (RAB4A Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mTFP1-Golgi-7

Plasmid

#55486

Purpose

Localization: GalT Targeting Sequence, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Golgi (B4GALT1 Human)

Use

Tags

mTFP1

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pNCST-AausFP4

Plasmid

#129502

Purpose

Expresses AausFP4 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP4

Use

Tags

Expression

Bacterial

Mutation

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBC2T-Ypet-FtnA

Plasmid

#108969

Purpose

expresses BC2-tag-Ypet-FtnA in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

FtnA (ftnA Escherichia coli)

Use

Tags

BC2-tag and Ypet

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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mTFP1-LaminB1-10

Plasmid

#55493

Purpose

Localization: Nuclear Envelope, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

LaminB1 (LMNB1 Human)

Use

Tags

mTFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCEP4-HA-Flag-natT1R2 a22-end_S212N

Plasmid

#113951

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Use

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

delete N-terminal 21 residues, S212N substitution

Promoter

CMV

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Arf6 T27N- Venus in pcDNA3.1

Plasmid

#90464

Purpose

RhoA mediated cell migration

Depositor

Narasimhan Gautam

Article

Castillo-Badillo et al ACS Synth Biol. 2020 Mar 16. doi: 10.102

Insert

Arf6 T27N (ARF6 )

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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GAL-p300-1000-1600

Plasmid

#89101

Purpose

mammalian expression of human p300 amino acids 1000-1600 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Use

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 1000-1600

Promoter

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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Hchk2 (kin-)-PUNI10

Plasmid

#41908

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

CHK2 (CHEK2 Human)

Use

Tags

Expression

Bacterial

Mutation

kinase dead (D347A)

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
pBEST-p70a-UTR1-Nterm 6xHis PL-T500

Plasmid

#92314

Purpose

Expression of NTerm 6xHis tagged protein L (PL) domain using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

B1 domain of protein L (PL)

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

p70a

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-REX-GECO0.9

Plasmid

#61247

Purpose

Expresses REX-GECO0.9 in mammalian cells

Depositor

Robert Campbell

Article

Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

Insert

REX-GECO0.9

Use

Tags

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1: P60R, V61W, R6…

Promoter

CMV

Available since

March 27, 2015

Availability

Academic Institutions and Nonprofits only
pmCherry-VIM-BC2T

Plasmid

#108966

Purpose

expresses mCherry-vimentin-BC2-tag in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

VIM (VIM Human)

Use

Tags

BC2T and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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p13xLexAOP2-IVS-Syn21-Voltron-p10

Plasmid

#119042

Purpose

LexAop2-driven expression of Voltron in Drosophila

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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