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Plasmid#161830PurposepRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterTEF1Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only
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pP3-GFP
Plasmid#53436PurposeControls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genomeDepositorInsertP3 promoter
UseSynthetic BiologyTagsnoneExpressionBacterialMutationnonePromoterP3Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
RFP Dynamin2 K44A
Plasmid#128153PurposeExpression of Dynamin 2 K44ADepositorInsertDynamin 2 (Dnm2 Rat)
UseTagsExpressionMammalianMutationK44APromoterAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pF hPGK mc2 IRES blast
Plasmid#206239PurposeFor expressing cloned exons as circular RNA.DepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
p306-BrdU-Inc
Plasmid#71792PurposeBrdU-incorporating plasmid with selectable marker URA3DepositorInsertsUseTagsExpressionYeastMutationPromoterADH1 and GPDAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
eMagB-iRFP-Mito
Plasmid#162250PurposeBlue-light dependent heterodimerization systemDepositorInserteMagB-iRFP-OMP25
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTet-ctl
Plasmid#136693PurposeControl for pTet-Gli2shRDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJRA-GW
Plasmid#209333PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-hfYFP
Plasmid#186522PurposeHyperfolder YFP fluorescent protein: bacterial expression plasmidDepositorInsertHyperfolder YFP
UseTags6xHisExpressionBacterialMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoteraraBAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MBP-yATOM_mCherry
Plasmid#209707PurposeMammalian expression of y-ATOM biosensor for mCherryDepositorInsertMBP-yATOM_mCherry
UseTagsMBPExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P shNFS1_1
Plasmid#102963PurposeSuppression of NFS1DepositorInsertshNFS1_1 (NFS1 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cherry-ICP0FxE-C3
Plasmid#134562PurposeExpresses Cherry-ICP0 fusion protein with a RING finger deletion (FXE)DepositorInsertICP0
UseTagsCherryExpressionMammalianMutationRING finger deletion (FXE)PromoterCMVAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-LSSmGFP
Plasmid#186534PurposeLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actinDepositorInsertLSSmGFP
UseTagsLifeActExpressionMammalianMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 Scr shRNA
Plasmid#59299Purposenon-targeting shRNADepositorInsertScr shRNA
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromotermouse U6Available sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-mhYFP
Plasmid#186530PurposeVisualization of the ER in cells using monomeric hyperfolder YFP (compatible with OSER assay)DepositorInsertMonomeric hyperfolder YFP
UseTagsCytochrome p450 first 29 animo acids (Oryctolagus…ExpressionMammalianMutationhfYFP-S147P/L195M/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
RFP Dynamin2 Wild Type
Plasmid#128152PurposeExpression of Dynamin 2DepositorInsertDynamin 2 (Dnm2 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pf hPGK mc2 IRES puro
Plasmid#206237PurposeFor expressing cloned exons as circular RNA.DepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCM271rfp
Plasmid#59774PurposepCMrfp with TrfA mutation (271R to C)DepositorInsertRFP
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMP4657
Plasmid#107773PurposeExpression of EGFPDepositorInsertpLacZ-EGFP in pME6010
UseTagspLacZ-EGFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
VAMP2-SNAP
Plasmid#105288PurposeVAMP2 tagged with SNAPDepositorInsertVAMP2-SNAP (Vamp2 Rat)
UseTagsSNAP tagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only