Skip to main content
Addgene
Showing: 4301 - 4320 of 5522 results

  1. pIgG1-2.1

    Plasmid
    #225831
    Purpose
    Mouse IgG1 chain specific for chicken gamma globulin (clone 2.1). Used to produce FLAG- and Strep2-tagged monoclonal antibodies for use as tag-specific ELISA standards.
    Depositor
    Gabriel Victora
    Article
    Schiepers et al Nature. 2023 Mar;615(7952):482-489. doi:
    Insert
    Mouse anti-CGG, IgG1 heavy chain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225831-plasmid-map-sequence-id-447625

    Enlarge

  3. TLNRD1(4H)-2T_pET47b

    Plasmid
    #224069
    Purpose
    Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations L191T/A225T, fused to an N-terminal hexa-histidine tag. HRV-3C cleavage site
    Depositor
    Ben Goult
    Article
    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003
    Insert
    tlnrd1 (TLNRD1 Human)
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    L191T/A225T double mutant; reduces affinity of TL…
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    224069-plasmid-map-sequence-id-443086

    Enlarge

  4. TLNRD1(4H)-2E_pET151

    Plasmid
    #224068
    Purpose
    Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations K192E/R233E, fused to an N-terminal hexa-histidine tag. TEV cleavage site
    Depositor
    Ben Goult
    Article
    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003
    Insert
    tlnrd1 (TLNRD1 Human)
    Use
    Tags
    6xHIS; V5
    Expression
    Bacterial
    Mutation
    K192E/R233E double mutant; prevents actin binding…
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    224068-plasmid-map-sequence-id-443085

    Enlarge

  11. pUC57-mini v2-HF24_F16

    Plasmid
    #195730
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#16 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195730-plasmid-map-sequence-id-385583

    Enlarge

  13. PLL5.0-Anillin ShRNA-GFP-AnillinA740D, E758K

    Plasmid
    #187274
    Purpose
    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K
    Depositor
    Alpha Yap
    Article
    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.
    Insert
    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Anillin A740D, E758K
    Promoter
    U6, 5'LTR
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    187274-plasmid-map-sequence-id-373247

    Enlarge

  20. pCIneo-AR-S256A

    Plasmid
    #171227
    Purpose
    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser256Ala (AR-S256A)
    Depositor
    Steven Balk
    Article
    Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10
    Insert
    human androgen receptor Ser256Ala mutant (AR Human)
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    AR-Ser256Ala (AR-S256A)
    Promoter
    CMV
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171227-plasmid-map-sequence-id-337969

    Enlarge

Showing: 4301 - 4320 of 5522 results