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Plasmid#194426PurposeExpresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiaeDepositorInsertCas9
UseTagsExpressionYeastMutationNAPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Spt2-GFP
Plasmid#115572PurposeExpresses yeast Spt2-GFP fusion proteinDepositorInsertSPT2
UseTagsGFPExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G3-Km
Plasmid#46817PurposeTEF1 and GAL10 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and GAL10Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G4-Ph
Plasmid#46820PurposeTEF1 and HXT7 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and HXT7Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGP8A-ccdB
Plasmid#83883PurposeDestination vector for integration at HIS3, expression from the ADH1 promoterDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB8742
Plasmid#126908PurposeTemplate for PCR amplification of pGPD1-pFBA1 double promoter biobrick for Yarrowia lipolyticaDepositorInsertpGPD1 and pFBA1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
p107.1
Plasmid#44517DepositorInsertHistone 4 N-terminus aa1-34
UseTagsGSTExpressionBacterialMutationaa1-34 of H4PromoterAvailable sinceMay 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAD
Plasmid#64747PurposeYeast expression plasmid: ACC1 (ser659ala, ser1157ala)DepositorInsertACC1 (ser659ala, ser1157ala)
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
p414ADH_Hsp82_CycTER
Plasmid#61712Purposeyeast plasmid with Hsp82DepositorInsert6xHis-Hsp82 (HSP82 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterADH1Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpAGO207-1251
Plasmid#117854PurposeExpressed KpAGO 207-1251 in E. coli under IPTG-inducible promotorDepositorInsertArgonaute
UseTags6xHis-SUMO encoded in vectorExpressionBacterialMutationmissing first 206 amino acid residuesPromoterT7Available sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCdeltaSS-ERE
Plasmid#108217PurposeYeast reporter gene with estrogen response element (ERE) upstream of CYC1 promoter driving lacZ expressionDepositorInsertERE-CYC1-lacZ
UseTagsExpressionYeastMutationPromoterCYC1Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYCTK019
Plasmid#176723PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Candida albicans (receiver part).DepositorArticleInsertste2Ca
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDZ207 (loxP KAN loxP 24xPP7SL MDN1)
Plasmid#35191DepositorInsertloxP KAN loxP 24xPP7SL MDN1
UseCre/LoxTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTCW002
Plasmid#83522Purposepheromone mediated destablised-GFP expression in yeastDepositorInsertpFUS1-yEGFPCLN2PEST
UseTagsExpressionYeastMutationPromoterpFUS1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS7
Plasmid#83774PurposeUseful for tagging yeast proteins with C-terminal Venus tag; G418 selectionDepositorInsertVenus
UseTagsVenusExpressionYeastMutationF46L, F64L, S65G, V68L, Q69M, S72A, Q80R, M153T, …PromoterAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
E2-Crimson-FKBP(L,V)
Plasmid#85000PurposeExpresses E2-Crimson-FKBP(L,V) in yeastDepositorInsertE2-Crimson-FKBP(LV)
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYCTK025
Plasmid#176729PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Lachancea mirantina (receiver part).DepositorArticleInsertste2Lm
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK028
Plasmid#176732PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Tetrapisispora phaffii (receiver part).DepositorArticleInsertste2Tp
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Sen1
Plasmid#99994PurposeShuttle yeast Sen1 into Gateway destination vectorDepositorInsertSen1 (SEN1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTH652-RLuc/maxFLuc
Plasmid#29698DepositorInsertFirefly Luciferase
UseTagsExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only