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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,052 results for: LEA

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Showing: 4281 - 4300 of 14052 results

  1. pMT-myl7-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80060
    Purpose
    Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Myl7 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80060-plasmid-map-sequence-id-152594

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  5. AD-003

    Plasmid
    #25502
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    AD-003 (NTMT1 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. IZ1181

    Plasmid
    #21049
    Depositor
    Tadahide Izumi
    Publication
    Busso et al Oncogene. 2009 Apr 2. 28(13):1616-25.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human MDM2 (MDM2 Human)
    Tags
    no tag
    Expression
    Mammalian
    Mutation
    no mutation. This is the wild-type human MDM2.
    Available since
    July 9, 2009
    Availability
    Academic Institutions and Nonprofits only

  12. pcDNA3-Akt-STOPS

    Plasmid
    #175006
    Purpose
    Akt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.
    Depositor
    Jin Zhang
    Publication
    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-Akt-STOPS
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    175006-plasmid-map-sequence-id-344970

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  13. pGBW-m4134093

    Plasmid
    #152116
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 ORF3a protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;V5
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152116-plasmid-map-sequence-id-300776

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  14. pGBW-m4134157

    Plasmid
    #152062
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp6
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 19, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    152062-plasmid-map-sequence-id-297885

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  18. pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE

    Plasmid
    #102986
    Purpose
    AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination
    Depositor
    Marco Tripodi
    Publication
    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306
    Inserts
    membrane-targeted SNAP protein after FLP-mediated recombination
    Nuclear-targeted eGFP after FLP-mediated recombination
    Use
    AAV; Adeno associated viral vector
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    102986-plasmid-map-sequence-id-200006

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Showing: 4281 - 4300 of 14052 results