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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,326 results for: LEA

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Showing: 4281 - 4300 of 12326 results

  1. pTE4565

    Plasmid
    #88903
    Purpose
    Expresses inactive/dead, humanized MbCpf1 nuclease
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hMbCpf1(D986A)
    Use
    CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    88903-plasmid-map-sequence-id-172594

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  2. PB-NES-ZapCV2

    Plasmid
    #222950
    Purpose
    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Publication
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NES-ZapCV2
    Tags
    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    222950-plasmid-map-sequence-id-450583

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  3. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid
    #69547
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69547-plasmid-map-sequence-id-122991

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  6. pICSL50025

    Plasmid
    #174589
    Purpose
    Level 0 C-terminal tag module carrying 6xHIS uncleavable
    Depositor
    Mark Banfield
    Insert
    6xHIS uncleavable
    Use
    Synthetic Biology
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174589-plasmid-map-sequence-id-344730

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  15. pZP06

    Plasmid
    #232320
    Purpose
    pZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E.
    Depositor
    Chenggang Wu
    Insert
    Pfdx-E
    Expression
    Bacterial
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232320-plasmid-map-sequence-id-462279

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  16. pST50Trc3-FLAGNDHFR

    Plasmid
    #63979
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Publication
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    FLAG; TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63979-plasmid-map-sequence-id-177385

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  19. pST50Trc2-STRNDHFR

    Plasmid
    #63960
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Publication
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Strep II (STR); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63960-plasmid-map-sequence-id-177366

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Showing: 4281 - 4300 of 12326 results