-
Plasmid#184958PurposeTest effect of a1/a2 length on editing fabH using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12
UseTagsExpressionBacterialMutationfabH donor G954APromoterT7/lacAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLY227
Plasmid#184148Purposeshort crRNA generator with spacer LEA2 with WT repeat regionDepositorInserts-crRNA-LEA2-WT
UseSynthetic BiologyTagsExpressionMutationPromoterPlux2Available sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.103
Plasmid#184988PurposeExpress Cas9-P2A-Eco1RTDepositorInsertCas9-P2A-Eco1RT
UseTagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.031
Plasmid#184968PurposeTest effect of extending -Eco2 a1/a2 in yeastDepositorInsertEco2: RT and ncRNA(extended), a1/a2 length: 29
UseTagsExpressionYeastMutationhuman codon optimized RT, a1/a2 length extended t…PromoterGal7Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.027
Plasmid#184964PurposeExpress -Eco1 RT and ncRNA in yeastDepositorInsertEco1: RT and ncRNA(wt), a1/a2 length: 12
UseTagsExpressionYeastMutationhuman codon optimized RTPromoterGal7Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.72
Plasmid#184984PurposeExpress Cas9/-Eco1 RT fusion, using linker1DepositorInsertCas9-fusion_linker1-Eco1RT
UseTagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.95
Plasmid#184986PurposeExpress Cas9/-Eco1 RT fusion, using linker2DepositorInsertCas9-fusion_linker2-Eco1RT
UseTagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.102
Plasmid#184987PurposeExpress -Eco1 RT-P2A-Cas9DepositorInsertEco1RT-P2A-Cas9
UseTagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.544
Plasmid#184961PurposeTest effect of a1/a2 length on editing murF using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22
UseTagsExpressionBacterialMutationmurF donor G1359A, a1/a2 length extended to 22 bpPromoterT7/lacAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.94
Plasmid#184985PurposeExpress -Eco1 RT/Cas9 fusion, using linker2DepositorInsertEco1RT-fusion_linker2-Cas9
UseTagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.006
Plasmid#184970PurposeExpress -Eco1 RT in yeastDepositorInsertIntegrating inducible cassette: Eco1 RT
UseTagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.005
Plasmid#184971PurposeExpress Cas9 in yeastDepositorInsertIntegrating inducible cassette: Cas9
UseTagsSV40NLSExpressionYeastMutationPromoterGal1-10Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.543
Plasmid#184960PurposeTest effect of a1/a2 length on editing murF using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
UseTagsExpressionBacterialMutationmurF donor G1359APromoterT7/lacAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.71
Plasmid#184983PurposeExpress -Eco1 RT/Cas9 fusion, using linker1DepositorInsertEco1RT-fusion_linker1-Cas9
UseTagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.545
Plasmid#184962PurposeTest effect of a1/a2 length on editing priB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
UseTagsExpressionBacterialMutationpriB donor G1069APromoterT7/lacAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
ND6-L1397-N_UGI-NES_right
Plasmid#187846Purposeexpresses the right-side half ND6-UGI-NES in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–NES–ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
ND6-L1397-N_UGI-NES_left
Plasmid#187845Purposeexpresses the left-side half ND6-UGI-NES in mammalian cellsDepositorInsertSOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–hivNES–SOD2 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB556
Plasmid#185108Purpose1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with S158A, F159A, F160ADepositorInsertPOM152
UseTagsGFPExpressionYeastMutation1st 200 amino acids (N-term + transmembrane domai…PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB553
Plasmid#185107Purpose1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with Y180L, Q182LDepositorInsertPOM152
UseTagsGFPExpressionYeastMutation1st 200 amino acids (N-term + transmembrane domai…PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB439
Plasmid#185093PurposeNMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion)DepositorInsertNMA111
UseTagsGFPExpressionYeastMutationGAL1::NMA111-GFPPromoterGAL1Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only