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Plasmid#134664PurposeExpresses EGFP-tagged endoplasmic reticulum markerDepositorInsertcalreticulin (CALR Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 18-413PromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEP-LAMP1-RFP
Plasmid#167970PurposeExpresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFPDepositorInsertLAMP1 (Lamp1 Rat)
UseTagsSEP and mRFPExpressionMammalianMutationD50EPromoterCMVAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBGreen-FIRE-puro
Plasmid#139195PurposeLuminescent reporter of ERK activityDepositorInsertCBG99
UseLuciferase and RetroviralTagsFra1 PEST domain fragment (FIRE) and nuclear loca…ExpressionMammalianMutationSee depositor comments belowPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-Cybt5-IRES-Puromycin
Plasmid#134869PurposeExpresses EGFP-tagged endoplasmic reticulum markerDepositorInsertcytochrome b5 type A (CYB5A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 1-98PromoterEF1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
DHFR-mNeon-GluA1
Plasmid#173009PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.DepositorInsertDHFR-mNeon-GluA1
UseTagsDHFR and mNeonExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
TfR-moxGFP-DHFR
Plasmid#173012PurposeFor light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).DepositorInsertTfR-moxGFP-DHFR
UseTagsDHFR and moxGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
mRuby2-Calreticulin-N-16
Plasmid#55892PurposeLocalization: Endoplasmic Reticulum, Excitation: 559, Emission: 600DepositorInsertCalreticulin (CALR Human)
UseTagsmRuby2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mRuby2-Calnexin-N-14
Plasmid#55891PurposeLocalization: Endoplasmic Reticulum, Excitation: 559, Emission: 600DepositorInsertCalnexin (CANX Human)
UseTagsmRuby2ExpressionMammalianMutationI474N in CalnexinPromoterCMVAvailable sinceMarch 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCIneoLuc-PP2A
Plasmid#128349PurposeVector for LUMIER assayDepositorInsertPP2A (PTPA Human)
UseTagsLuciferaseExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-TetR
Plasmid#103813PurposeBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationTetR: A4G (M2V)PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry1-ER-5
Plasmid#57174PurposeLocalization: Endoplasmic Reticulum, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertER (CALR Human)
UseTagsPA-mCherry1ExpressionMammalianMutationER Signal PeptidePromoterCMVAvailable sinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
ER-CanlonicSF
Plasmid#178343PurposeTo explore endoplasmic reticulum lactate dynamicsDepositorInsertER-CanlonicSF
UseTagsER Retention Signal, ER Signal Exon 1, and ER Sig…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
mPapaya-Calnexin-N-14
Plasmid#56343PurposeLocalization: Endoplasmic Reticulum, Excitation: 530, Emission: 541DepositorInsertCalnexin (CANX Human)
UseTagsmPapayaExpressionMammalianMutationI474N in CalnexinPromoterCMVAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC19-J6LE60
Plasmid#103117PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertJ6LE60(Cas9 coding gene from Rhodovulum sp. PH10)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Q0P897
Plasmid#103120PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertQ0P897(Cas9 coding gene from Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-D3NT09
Plasmid#103074PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertD3NT09(Cas9 coding gene from Azospirillum sp. (strain B510))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
mPapaya1-Calnexin-N-14
Plasmid#56350PurposeLocalization: Endoplasmic Reticulum, Excitation: 530, Emission: 541DepositorInsertCalnexin (CANX Human)
UseTagsmPapaya1ExpressionMammalianMutationI474N in CalnexinPromoterCMVAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV/EF1a::DIO-iLMO2
Plasmid#129482PurposeAAV2 transfer plasmid for Cre-dependent expression of iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemicalDepositorInsertiLMO2
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-A7HP89
Plasmid#103082PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertA7HP89(Cas9 coding gene from Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG RpH-LAMP1-3xFLAG
Plasmid#163018Purposeexpresses ratiometric sensor of lysosomal lumenal pHDepositorInsertRpH-LAMP1-3xFLAG (Lamp1 Mouse)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only