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Showing: 4261 - 4280 of 37861 results

  2. KC1G3_HUMAN_D0

    Plasmid
    #79685
    Purpose
    This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    Markus Seeliger, John Chodera, Nicholas Levinson
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    KC1G3 (CSNK1G3 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. KCC2G_HUMAN_D0

    Plasmid
    #79711
    Purpose
    This plasmid encodes the kinase domain of KCC2G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    Markus Seeliger, John Chodera, Nicholas Levinson
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    KCC2G (CAMK2G Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Use
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182287-plasmid-map-sequence-id-363047

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  15. pGL403

    Plasmid
    #119953
    Purpose
    Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102
    Inserts
    gpps
    limS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-terminal truncation of 56 amino acid residues (…
    Promoter
    Available since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    119953-plasmid-map-sequence-id-238006

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  18. DiLiCre 2.0

    Plasmid
    #198752
    Purpose
    A Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    Cre-PhoCI-ERT2-ERT2
    Use
    Lentiviral
    Tags
    Cre, kept in a photocleavable site, followed by 2…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TRE3GV
    Available since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198752-plasmid-map-sequence-id-397647

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  20. pAAV-CAG-H2BmGreenLantern

    Plasmid
    #177332
    Purpose
    To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.
    Depositor
    Murray Blackmore, Pantelis Tsoulfas, Gregory Petsko
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    H2B (Hist1h2bq Rat)
    Use
    AAV
    Tags
    mGreenLantern
    Expression
    Mammalian
    Mutation
    This fusion protein was optimized to the Human co…
    Promoter
    CAG
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177332-plasmid-map-sequence-id-353323

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Showing: 4261 - 4280 of 37861 results