Skip to main content
Addgene
Showing: 4241 - 4260 of 42536 results

  1. pBS/U6/RNAi gamma-tubulin shRNA mouse

    Plasmid
    #70666
    Purpose
    Reduced the expression of gamma-tubulin in murine cells
    Depositor
    Maria Alvarado-Kristensson
    Article
    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1
    Insert
    5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´ (Tubg1, Tubg2 Mouse)
    Use
    U6 promoter
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70666-plasmid-map-sequence-id-133021

    Enlarge

  2. CoxVIIIx2-OeNL(Ca2+)_18μ-pcDNA3

    Plasmid
    #111924
    Purpose
    Orange color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of OeNL(Ca2+)_18μ
    Depositor
    Takeharu Nagai
    Article
    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/
    Insert
    OeNL(Ca2+)_18μ
    Use
    Tags
    CoxVIIIx2
    Expression
    Mammalian
    Mutation
    E67D, E104D, D133E at CaM
    Promoter
    CMV
    Available since
    June 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    111924-plasmid-map-sequence-id-214007

    Enlarge

  16. pUDP010

    Plasmid
    #101166
    Purpose
    E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101166-plasmid-map-sequence-id-199274

    Enlarge

  18. pSC51

    Plasmid
    #104825
    Purpose
    CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter
    Depositor
    Robert Stupar
    Article
    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10
    Insert
    Glyma.12g075700, Glyma.11g145900
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    104825-plasmid-map-sequence-id-203148

    Enlarge

  19. pCIS-rPALcc

    Plasmid
    #145804
    Purpose
    expresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cells
    Depositor
    Betty Eipper, Richard Mains
    Article
    Kolhekar et al Biochemistry. 2002 Oct 15;41(41):12384-9
    Insert
    rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed serine 767 to alanine to prevent glycosyl…
    Promoter
    CMV
    Available since
    June 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    145804-plasmid-map-sequence-id-299336

    Enlarge

Showing: 4241 - 4260 of 42536 results