Addgene

We narrowed to 14,719 results for: LEA

Showing: 4241 - 4260 of 14719 results

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pSLS.545

Plasmid

#184962

Purpose

Test effect of a1/a2 length on editing priB using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12

Use

Tags

Expression

Bacterial

Mutation

priB donor G1069A

Promoter

T7/lac

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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ND6-L1397-N_UGI-NES_right

Plasmid

#187846

Purpose

expresses the right-side half ND6-UGI-NES in mammalian cells

Depositor

Chengqi Yi

Article

Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

Insert

COX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–NES–ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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ND6-L1397-N_UGI-NES_left

Plasmid

#187845

Purpose

expresses the left-side half ND6-UGI-NES in mammalian cells

Depositor

Chengqi Yi

Article

Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

Insert

SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–hivNES–SOD2 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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KBB556

Plasmid

#185108

Purpose

1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with S158A, F159A, F160A

Depositor

Ken Belanger

Article

Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

Insert

POM152

Use

Tags

GFP

Expression

Yeast

Mutation

1st 200 amino acids (N-term + transmembrane domai…

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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KBB553

Plasmid

#185107

Purpose

1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with Y180L, Q182L

Depositor

Ken Belanger

Article

Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

Insert

POM152

Use

Tags

GFP

Expression

Yeast

Mutation

1st 200 amino acids (N-term + transmembrane domai…

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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KBB439

Plasmid

#185093

Purpose

NMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

GAL1::NMA111-GFP

Promoter

GAL1

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB345

Plasmid

#185078

Purpose

rfa3D46 truncation fused to GFP

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA3

Use

Tags

GFP

Expression

Yeast

Mutation

Rfa3 truncation aa 1-46

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB419

Plasmid

#185089

Purpose

Swi6L-GFP expressing N-term 273 amino acids of Swi6

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Use

Tags

GFP

Expression

Yeast

Mutation

Swi6 truncation at aa 273

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB462

Plasmid

#185095

Purpose

NMA111-GFP with NLS1 and NLS2 mutated under control of GAL1 promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

GAL1::nma111Dnls1Dnls2-GFP

Promoter

GAL1

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB354

Plasmid

#185082

Purpose

Swi6L-GFP expressin N-term 273 amino acids of Swi6

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Use

Tags

GFP

Expression

Yeast

Mutation

Swi6 truncation aa 1-273

Promoter

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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KBB349

Plasmid

#185080

Purpose

rfa2D248 truncation fused to GFP

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA2

Use

Tags

GFP

Expression

Yeast

Mutation

Rfa2 truncation aa 1-247

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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KBB353

Plasmid

#185081

Purpose

Swi6M-GFP expressing N-term 181 amino acids of Swi6

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Use

Tags

GFP

Expression

Yeast

Mutation

Swi6 truncation aa 1-181

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEGPN

Plasmid

#187482

Purpose

Expression vector - GFP

Depositor

Lewis Cantley

Article

Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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KBB484

Plasmid

#185132

Purpose

SWI6-GFP full length under control of GAL1 promotion

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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KBB486

Plasmid

#185133

Purpose

Full length CRM1 fused to binding domain of two-hybrid plasmid

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

CRM1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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KBB491

Plasmid

#185134

Purpose

Full length SWI6 fused to activation domain of two-hybrid plasmid

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pVH9-Tier1-PhCMV-3xNLS

Plasmid

#169478

Purpose

Tier-1 vector encoding PhCMV-driven triple nuclear localization signal expression (PhCMV-3xNLS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nuclear localization signal

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVH8-Tier1-PhCMV-NLS

Plasmid

#169477

Purpose

Tier-1 vector encoding PhCMV-driven nuclear localization signal expression (PhCMV-NLS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nuclear localization signal

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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TDRD3:ABC006-C02:C220502

Plasmid

#183982

Purpose

Baculovirus expression for structure determination. May not contain entire coding region of gene.

Depositor

Cheryl Arrowsmith

Insert

TDRD3-anti-TDRD3 FAB (TDRD3 Human)

Use

Tags

anti-TDRD3 FAB HC: N-terminal STII leader peptid…

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pHBT-Mb(GDx-Clo_L10)

Plasmid

#183406

Purpose

Expresses Monobody GDx-Clo_L10 in E. coli

Depositor

Shohei Koide

Article

Kermani et al Nat Commun. 2020 Nov 27;11(1):6064. doi:

Insert

His tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)

Use

Tags

His tag-Avi tag-TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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