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pSEM246 - mosTI - unc-119 - MCS

Plasmid

#182343

Purpose

Empty cloning vector for mosTI(unc-119). MCS or Golden Gate (BsaI: tgcc - MCS - gagc)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGEP179_pX330K

Plasmid

#137882

Purpose

Entry vector for multi-gRNA cloning

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pmtb-t7-alpha-bungarotoxin

Plasmid

#69542

Purpose

For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

Depositor

Sean Megason

Article

Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

Insert

alpha-bungarotoxin

Use

Tol2, beta-actin promoter, sp6,t7 for zebrafish

Tags

Expression

Mutation

Promoter

beta-actin, sp6, t7

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec23A

Plasmid

#66609

Purpose

GFP fusion of Sec23A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec23A (SEC23A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO-cell-tagRFP

Plasmid

#185598

Purpose

Lentiviral construct for expression of tagRFP-T

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

cell-tagRFP-T

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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PB-Ostir-neo

Plasmid

#161973

Purpose

Expresses Ostir and G418 resistance gene in mammalian cells

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

Ostir-3xHA-p2a-neoR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Taq DNA Ligase

Plasmid

#178798

Purpose

Inducible expression of Taq DNA Ligase in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Ligase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pACAGW-ChR2-Venus-AAV

Plasmid

#20071

Purpose

AAV-mediated expression of ChR2-Venus for neuronal excitation

Depositor

Karel Svoboda

Article

Leopoldo et al Nature. 2009 Jan 18.

Has Service

AAV1 and AAV9

Insert

Channelrhodopsin-2 (CHLRE_02g085257v5 Chlamydomonas reinhardtii)

Use

AAV

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CAGG

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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mCherry- SEC61B

Plasmid

#121160

Purpose

Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

SEC61B (SEC61B )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGFPmAID

Plasmid

#174213

Purpose

GFP::mAID donor backbone

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2 Notch1 Full Length-6MT

Plasmid

#41728

Depositor

Raphael Kopan

Article

Schroeter et al Nature. 1998 May 28;393(6683):382-6.

Insert

Notch-1 Full Length (Notch1 Mouse)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

L1997V; Contains murine Notch-1 protein up to ami…

Promoter

Available since

Dec. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pHH0103_DNAJB1_DNAJ

Plasmid

#109918

Purpose

Protein expression and purification of DNAJB1_DNAJ

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DNAJB1_DNAJ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-LIC

Plasmid

#120247

Purpose

For LIC cloning with N-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro GFP shRNA

Plasmid

#10675

Depositor

William Hahn

Article

Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

Insert

GFP shRNA

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-LIC-V5

Plasmid

#120248

Purpose

For LIC cloning with C-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-TC10

Plasmid

#23232

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

TC10 (RHOQ Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pMF1706

Plasmid

#125582

Purpose

Expresses peroxisomal roGFP2 (po-roGFP2) in mammalian cells

Depositor

Marc Fransen

Article

Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. d

Insert

po-roGFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAC-GAMMAipi

Plasmid

#53278

Purpose

Has ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and crtYm gene of marine bacterium P99-3. and produces gamma-carotene in Escherichia coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

Insert

crtYm

Use

Low copy number bacterial cloning vector

Tags

Expression

Mutation

Promoter

endogenous promoter

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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mCarmine in pcDNA3

Plasmid

#109486

Purpose

mammalian expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

Depositor

Oliver Griesbeck

Article

Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

Insert

mCarmine

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pKvenus-Scribble

Plasmid

#58738

Purpose

Expresses Venus-tagged human Scribble protein

Depositor

Ian Macara

Article

Tatsis et al J Biol Chem. 1998 Dec 18. 273(51):34631-

Insert

Scribble (SCRIB Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2014

Availability

Academic Institutions and Nonprofits only

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