Addgene

We narrowed to 14,102 results for: LEA

Showing: 4221 - 4240 of 14102 results

Results per page:

KBB486

Plasmid

#185133

Purpose

Full length CRM1 fused to binding domain of two-hybrid plasmid

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

CRM1

Use

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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KBB491

Plasmid

#185134

Purpose

Full length SWI6 fused to activation domain of two-hybrid plasmid

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Use

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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LDB204

Plasmid

#185426

Purpose

Bacterial expression of FG-repeat domain of NUP1 nucleoporin as GST-Nup1-Rep fusion

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NUP1

Use

Tags

GST

Expression

Bacterial

Mutation

Nup1 FXFG-repeats domain expressed

Promoter

Availability

Academic Institutions and Nonprofits only

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pAF-HdrB3

Plasmid

#184908

Purpose

carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

Depositor

Radhakrishnan Mahadevan

Article

Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

Insert

Cas9-sgRNA-homology arms

Use

CRISPR; Broad-host-range

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSLS.491

Plasmid

#184956

Purpose

Test effect of a1/a2 length on editing rpoB using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12

Use

Tags

Expression

Bacterial

Mutation

rpoB donor T1534C

Promoter

T7/lac

Availability

Academic Institutions and Nonprofits only

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pSLS.492

Plasmid

#184957

Purpose

Test effect of a1/a2 length on editing rpoB using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22

Use

Tags

Expression

Bacterial

Mutation

rpoB donor T1534C, a1/a2 length extended to 22 bp

Promoter

T7/lac

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLS.539

Plasmid

#184958

Purpose

Test effect of a1/a2 length on editing fabH using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12

Use

Tags

Expression

Bacterial

Mutation

fabH donor G954A

Promoter

T7/lac

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLS.540

Plasmid

#184959

Purpose

Test effect of a1/a2 length on editing fabH using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 22

Use

Tags

Expression

Bacterial

Mutation

fabH donor G954A, a1/a2 length extended to 22 bp

Promoter

T7/lac

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLS.543

Plasmid

#184960

Purpose

Test effect of a1/a2 length on editing murF using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12

Use

Tags

Expression

Bacterial

Mutation

murF donor G1359A

Promoter

T7/lac

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLS.544

Plasmid

#184961

Purpose

Test effect of a1/a2 length on editing murF using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22

Use

Tags

Expression

Bacterial

Mutation

murF donor G1359A, a1/a2 length extended to 22 bp

Promoter

T7/lac

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLS.545

Plasmid

#184962

Purpose

Test effect of a1/a2 length on editing priB using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12

Use

Tags

Expression

Bacterial

Mutation

priB donor G1069A

Promoter

T7/lac

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLS.546

Plasmid

#184963

Purpose

Test effect of a1/a2 length on editing priB using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 22

Use

Tags

Expression

Bacterial

Mutation

priB donor G1069A, a1/a2 length extended to 22 bp

Promoter

T7/lac

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.027

Plasmid

#184964

Purpose

Express -Eco1 RT and ncRNA in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RT and ncRNA(wt), a1/a2 length: 12

Use

Tags

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal7

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.028

Plasmid

#184965

Purpose

Test effect of extending -Eco1 a1/a2 in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RT and ncRNA(extended), a1/a2 length: 27

Use

Tags

Expression

Yeast

Mutation

human codon optimized RT, a1/a2 length extended t…

Promoter

Gal7

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.031

Plasmid

#184968

Purpose

Test effect of extending -Eco2 a1/a2 in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco2: RT and ncRNA(extended), a1/a2 length: 29

Use

Tags

Expression

Yeast

Mutation

human codon optimized RT, a1/a2 length extended t…

Promoter

Gal7

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.006

Plasmid

#184970

Purpose

Express -Eco1 RT in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Integrating inducible cassette: Eco1 RT

Use

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.005

Plasmid

#184971

Purpose

Express Cas9 in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Integrating inducible cassette: Cas9

Use

Tags

SV40NLS

Expression

Yeast

Mutation

Promoter

Gal1-10

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.71

Plasmid

#184983

Purpose

Express -Eco1 RT/Cas9 fusion, using linker1

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1RT-fusion_linker1-Cas9

Use

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.72

Plasmid

#184984

Purpose

Express Cas9/-Eco1 RT fusion, using linker1

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker1-Eco1RT

Use

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.94

Plasmid

#184985

Purpose

Express -Eco1 RT/Cas9 fusion, using linker2

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1RT-fusion_linker2-Cas9

Use

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Availability

Academic Institutions and Nonprofits only

Enlarge