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Plasmid#175380PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, URA3-selected.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only
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CAA 659
Plasmid#108684Purposein vitro mRNA transcriptionDepositorInsertCAA repeat mRNA
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pASZ-C-DM
Plasmid#111235Purposeyeast expression vectorDepositorInsertccdB-cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pASZ-CN-DM
Plasmid#111234Purposeyeast expression vectorDepositorInsertccdB-cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Ckb1
Plasmid#99981PurposeShuttle yeast Ckb1 into Gateway destination vectorDepositorInsertCkb1 (CKB1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Ckb2
Plasmid#99982PurposeShuttle yeast Ckb2 into Gateway destination vectorDepositorInsertCkb2 (CKB2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Utp11
Plasmid#99984PurposeShuttle yeast Utp11 into Gateway destination vectorDepositorInsertUtp11 (UTP11 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Pfa1
Plasmid#99999PurposeShuttle yeast Pfa1 into Gateway destination vectorDepositorInsertPfa1 (SQS1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Cka1
Plasmid#99979PurposeShuttle yeast Cka1 into Gateway destination vectorDepositorInsertCka1 (CKA1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pDS3-X4
Plasmid#70140PurposeYeast chr. integration vector with DS3 selection markerDepositorInsertDS3
UseTagsExpressionYeastMutationPromoterAgTEF1Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZHY566
Plasmid#50608PurposeTALEN expression vector for yeast, N152,C18 architectureDepositorTypeEmpty backboneUseTALENTagsExpressionYeastMutationPromoterTEFAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZHY565
Plasmid#50607PurposeTALEN expression vector for yeast, N152,C18 architectureDepositorTypeEmpty backboneUseTALENTagsExpressionYeastMutationPromoterTEFAvailable sinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1deltaC(GiVL-1)
Plasmid#32032DepositorInsertDCR1(15-355)
UseTagsHis6-SUMOExpressionBacterialMutationH123-N143 replaced by P642-Y645 of GiDicerPromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1(1-384)
Plasmid#32031DepositorInsertDCR1(1-384)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1deltaC(GiVL-2)
Plasmid#32034DepositorInsertDCR1(15-355)
UseTagsHis6-SUMOExpressionBacterialMutationN195-M215 replaced by P697-D714 of GiDicerPromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1deltaC(E147Q/D151N)
Plasmid#32024DepositorInsertDCR1(15-355)
UseTagsHis6-SUMOExpressionBacterialMutationE147Q/D151NPromoterT7Available sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAS
Plasmid#60847PurposeExpresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeastDepositorInsertsS. pyogenese Cas9
RNA pol III promoter (tRNA-Tyr)
hepatitis delta virus ribozyme, genomic
sgRNA
UseCRISPR and Synthetic BiologyTagsNLS/His8 and TTT 3' extension prior to sgRNAExpressionBacterial and YeastMutationL4 is UUCG tetraloop and guide targets LYP1 (CATA…PromoterAvailable sinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-dCas9-VPR
Plasmid#63801PurposepAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vectorDepositorInsertdCas9-VPR
UseCRISPR and Synthetic BiologyTagsVPR (VP64-p65-Rta)ExpressionYeastMutationPromoterGPDAvailable sinceApril 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ2
Plasmid#197415PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-2 σ2
UseTagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationencodes R42G substitution, contains silent substi…PromoterADH1 and MET25Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only