-
Plasmid#75447PurposePlasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1B
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT1D
Plasmid#75448PurposePlasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1D
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT1E
Plasmid#75449PurposePlasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1E
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTargetF
Plasmid#62226PurposeConstitutive expression of sgRNA without donor editing template DNADepositorInsertsgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpij23119Available sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRNA-CleanCap-mNeonGreen
Plasmid#188248PurposeTemplate plasmid to transcribe mRNA encoding mNeonGreenDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGTR
Plasmid#63143PurposePCR template to construct polycistronic tRNA-gRNADepositorInsertgRNA-tRNA fusion
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTKLP-tetA
Plasmid#71325PurposetetA landing pad template plasmidDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRKH2
Plasmid#71312PurposeTheta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.DepositorTypeEmpty backboneUseE. coli-gram positive bacteria shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDB4581
Plasmid#171124PurposeA kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus.DepositorInsert3_sAID
UseOtherTagsExpressionMutationnon applicablePromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
His6-SpyCatcher-mi3
Plasmid#113043PurposeExpresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccinationDepositorInsertHis6-SpyCatcher-mi3
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAL-pck_NT
Plasmid#74069PurposepAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecRDepositorInsertsgRNA pck (NT)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCB591
Plasmid#141096PurposeE. coli-Lactobacilli shuttle vector for recombineering template cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP Donor
Plasmid#202689PurposeCLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.DepositorInsertsCopGFP
mCherry
UseTagsExpressionMutationPromoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-116:PCNA-mEGFP
Plasmid#170818PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human PCNADepositorInsertPCNA Homology Arms with mEGFP-linker (PCNA Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Monomer NH_V2
Plasmid#37527Purposetemplate for PCR amplification of the monomer used for assembly of the DNA-binding tandem repeat region of the TALE.DepositorInsertMonomer NH
UseTagsExpressionMutationPromoterAvailable sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA CUP1p-NuiHA kanMX
Plasmid#131169PurposePCR template for genomic ScCUP1 promoter regulated N-terminal Nui-HA prey fusion, kanMX marker confers resistance to geneticin in S. cerevisiaeDepositorTypeEmpty backboneUseTagsNui-HAExpressionYeastMutationPromoterAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMLS256
Plasmid#73715PurposeSapTrap destination vector for building combined sgRNA expression + repair template vectorsDepositorInsertpU6::sgRNA
UseCRISPRTagsExpressionWormMutationSapI insertion sitePromoterC. elegans U6 snRNA pol III promoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL-pgi_NT
Plasmid#74067PurposepAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecRDepositorInsertsgRNA pgi (NT)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Rluc-BoxB-A114-N40
Plasmid#50562PurposeTemplate DNA for in vitro transcription using T7 polymeraseDepositorInsertLuciferase-BoxB-A114-N40
UseCloning vectorTagsExpressionMutationPromoterlacAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pECM1
Plasmid#75445PurposePlasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only