-
Plasmid#122568PurposeMAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)DepositorInsertbeta-lactamase/sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBad-Xis/Int Reset Flipper
Plasmid#38207DepositorInsertsBxb1-Excisionase
Bxb1 Integrase
UseSynthetic Biology; Biobrick cloning vectorTagsExpressionMutationPromoterpBADAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH1
Plasmid#84451Purposeshuttle plasmid for sarA P1-dsRED expressionDepositorInsertDsRed.T3(DNT)
UseShuttle vector e.coli-s.aureus, expression of sgf…TagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACλCI-β-flap (831-1057)
Plasmid#53733PurposeExpression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertλCI-β-flap fusion
UseTagsExpressionBacterialMutationPromoterlac UV5Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZmUBQ:GW
Plasmid#132358PurposeExpression of CDS from the Zea mays ubiquitin promotorDepositorTypeEmpty backboneUseTagsYFPExpressionPlantMutationPromoterpZmUBQAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbA4c-RFP
Plasmid#35299DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterProEAvailable sinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJB007
Plasmid#98995PurposeExpress FtsI tagged with tagRFP-t in E coliDepositorInsertFtsI
UseTagsTagRFP-TExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRPF185
Plasmid#106367PurposeC. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusADepositorInsertTerm(fdx)-Ptet-gusA-Term(slpA) (gusA )
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationcodon optimised for C. difficilePromoterPtetAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF-I-GFP GX
Plasmid#45443DepositorTypeEmpty backboneUseTagsIRES EGFPExpressionMammalianMutationPromoterhuman EF1αAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIM1708-surT-PsurP-agaN-pNW33N
Plasmid#44009DepositorInsertsurT-PsurP-agaN
UseTagsExpressionBacterialMutationDeleted EcoRI site (synonymous change, C to T at …PromoterAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
1455_pDEST_miniTol2_R4-R2_MCS
Plasmid#171794PurposepDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a Multiple Cloning Site for selection marker insertion.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRETgc-2in1-CC
Plasmid#105115PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmGFP/mCherryExpressionPlantMutationPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbB5c-GFP
Plasmid#35353DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS3
Plasmid#127128DepositorInsertRPS3 (RPS3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKS014
Plasmid#65467PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidaseDepositorInsertNtag-beta galactosidase
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterpl-TetOAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR
Plasmid#47643Purposewt esaR gene under control of Plac in pACYC184DepositorInsertPlac-esaR-terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRATIO3212
Plasmid#141311PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmScarlet-I and mVenusExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbA0c-RFP
Plasmid#35373DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRATIO3267
Plasmid#141312PurposeGateway cloning compatible dual bioluminescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsgLUC and redLUCExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-APLO
Plasmid#112805Purposegateway cloning destination vector to express LexAop2 driven transgene in DrosophilaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only