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Plasmid#148364PurposeBacterial Expression of DmMextli_577-640-M605DDepositorInsertDmMextli_577-640-M605D (mxt Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmMextli_577-640-Y581AL586AL587A-GB1_AB
Plasmid#148365PurposeBacterial Expression of DmMextli_577-640-Y581AL586AL587ADepositorInsertDmMextli_577-640-Y581AL586AL587A (mxt Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmMextli_577-640-YLLILtoAAAAA-GB1_AB
Plasmid#148366PurposeBacterial Expression of DmMextli_577-640-YLLILtoAAAAADepositorInsertDmMextli_577-640-YLLILtoAAAAA (mxt Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot4-dsRNAres_Q
Plasmid#147339PurposeInsect Expression of DmNot4-dsRNAresDepositorInsertDmNot4-dsRNAres (CG31716 Fly)
UseTagsExpressionInsectMutation7 silent mutations and 5 non silent mutations G20…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1_1-550-siRNAres_L
Plasmid#146859PurposeInsect Expression of DmPABPC1_1-550-siRNAresDepositorInsertDmPABPC1_1-550-siRNAres (pAbp Fly)
UseTagsExpressionInsectMutationone non silent mutation G26S, and six silent muta…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1_90-635-siRNAres-V5His6_L
Plasmid#146860PurposeInsect Expression of DmPABPC1_90-635-siRNAresDepositorInsertDmPABPC1_90-635-siRNAres (pAbp Fly)
UseTagsExpressionInsectMutationfive silent mutations compared to the sequence gi…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-del91-164-V5His6_L
Plasmid#146861PurposeInsect Expression of DmPABPC1_del91-164DepositorInsertDmPABPC1_del91-164 (pAbp Fly)
UseTagsExpressionInsectMutationone non silent mutation G26S, and five silent mut…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-del183-258-siRNAres-V5His6_L
Plasmid#146862PurposeInsect Expression of DmPABPC1_del183-258-siRNAresDepositorInsertDmPABPC1_del183-258-siRNAres (pAbp Fly)
UseTagsExpressionInsectMutationone non silent mutation G26S, and seven silent mu…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-del288-359-siRNAres-V5His6_L
Plasmid#146863PurposeInsect Expression of DmPABPC1_del288-359-siRNAresDepositorInsertDmPABPC1_del288-359-siRNAres (pAbp Fly)
UseTagsExpressionInsectMutationone non silent mutation G26S, and six silent muta…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-del364-550-siRNAres-V5His6_L
Plasmid#146864PurposeInsect Expression of DmPABPC1_del364-550-siRNAresDepositorInsertDmPABPC1_del364-550-siRNAres (pAbp Fly)
UseTagsExpressionInsectMutationone non silent mutation G26S, and five silent mut…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
YW347_Ptbb2UTR-loxP-RFP-miniMOS
Plasmid#196064Purposea plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.DepositorInsertsmyo2p driven tagRFP
NeoR
Minimal Mos1
UseTagsExpressionWormMutationPromotermyo-2Available sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676-GB1_AD
Plasmid#148512PurposeBacterial Expression of DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676DepositorInsertDmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676 (eIF4G1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAS-dsarm(E,K450R)::flag
Plasmid#187882PurposeGal4/UAS expression of dSarm(isoE,K450R)::FLAGDepositorInsertdSarm isoform E (Sarm )
UseTagsFLAGExpressionMutationK450RPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS-dsarm(E,K450R)
Plasmid#187881PurposeGal4/UAS expression of dSarm(isoE,K450R)DepositorInsertdSarm isoform E (Sarm )
UseTagsExpressionMutationK450RPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot4-dsRNAres_Q
Plasmid#147340PurposeInsect Expression of DmNot4-dsRNAresDepositorInsertDmNot4-dsRNAres (CG31716 Fly)
UseTagsExpressionInsectMutation7 silent mutations and 6 non silent mutations G20…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1710-2505_J
Plasmid#146702PurposeInsect Expression of DmNot1_1710-2505DepositorInsertDmNot1_1710-2505 (Not1 Fly)
UseTagsExpressionInsectMutationtwo non silent mutations (A1747T, G1999S) and one…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRR-C421-G2304D
Plasmid#182845Purposemutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sitesDepositorInsertTRR 2011-2431 (trr Fly)
UseTagsGSTExpressionBacterialMutationmutation G2304D (GGC/GAC)PromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-CBP(1-287)
Plasmid#182839PurposeCBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)DepositorInsertCBP1-287
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC(75-228)-H6
Plasmid#183779PurposeExpress PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP pull-down assays.DepositorInsertPolycomb (Pc Fly)
UseTags6 His Tag at the C-terminusExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC(75-390)-H6
Plasmid#183781PurposeExpress PC(75-390)-H6 in E. coli. PC(75-390)-H6 was used for GST-CBP pull-down assaysDepositorInsertPolycomb (w Fly)
UseTags6 His tag at the C-terminusExpressionBacterialMutation6 His tag at the C-terminusPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only