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Plasmid#50478PurposeGq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoterDepositorHas ServiceAAV5InserthM3D(Gq)-mCherry
UseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ZNF598-TEV-3xFLAG
Plasmid#105690PurposeExpresses C terminally 3xFLAG tagged ZNF598 proteinDepositorInsertZNF598 (ZNF598 Human)
UseTagsTEV-3xFLAGExpressionMammalianMutationPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Halo-TOMM20-N-10
Plasmid#123284PurposeLocalization: Outer Mitochondrial MembraneDepositorInsertTOMM20 (TOMM20 Human)
UseTagsHaloTag7ExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPF215
Plasmid#106377PurposeC. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposonDepositorInsertPtet-himar1-Ter(slpA)-transposon
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationCodon optimised for C. difficilePromoterPtetAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_iRFP670 sgBFP
Plasmid#192478PurposeAll-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3DepositorInsertSpCas9
UseTagsT2A iRFP670ExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.PI.EGFP.WPRE.bGH (AAV2)
Viral Prep#105530-AAV2PurposeReady-to-use AAV2 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCMVTagsEGFPAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)
Plasmid#185912PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpRY-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM3D(Gq)-mCherry (AAV8)
Viral Prep#50474-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA. hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherryAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS
Plasmid#71396Purpose2Gi2R assayDepositorInserts2xGFP:NES
2xRFP:NLS
UseLentiviralTagsIRESExpressionMammalianMutationPromoterEF1alpha and IRESAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GfaABC1D.PI.Lck-GFP.SV40 (AAV5)
Viral Prep#105598-AAV5PurposeReady-to-use AAV5 particles produced from pAAV.GfaABC1D.PI.Lck-GFP.SV40 (#105598). In addition to the viral particles, you will also receive purified pAAV.GfaABC1D.PI.Lck-GFP.SV40 plasmid DNA. GfaABC1D-driven GFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterGfaABC1DTagsGFPAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB700
Plasmid#64046PurposeLentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent proteinDepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6, CAGGSAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 phiC31
Plasmid#68310PurposeIn vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).DepositorInsertphiC31
UseRna transcriptionTagsExpressionMutationPromoterCMVAvailable sinceAug. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
HC-M
Plasmid#173481PurposeAn rrnB P1-based GFP ATP reporter in E. coliDepositorInsertGFP-mut2
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialMutationPromoterrrnB P1Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCE-hUL
Plasmid#41855PurposeNon-integrating (episomal) expression of human L-MYC and LIN28DepositorUseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
p5154
Plasmid#175390PurposeHigh Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)DepositorTypeEmpty backboneUseMouse Targeting; Cloning vector, to be used to de…TagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV9)
Viral Prep#162381-AAV9PurposeReady-to-use AAV9 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (#162381). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
MoClo Yeast Secretion and Display (YSD) Toolkit
Plasmid Kit#1000000240PurposeThe YSD Toolkit is a collection of 34 plasmids that can be used in conjunction with the MoClo Yeast Toolkit (YTK) to optimize secretion and cell surface display of proteins of interest in yeast.DepositorAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-A2A-adenosine receptor
Plasmid#66995PurposeA2A-adenosine receptor tagged with HaloTag on amino terminusDepositorInsertHaloTag-A2A-adenosine receptor (ADORA2A Human)
UseTagsHuman Growth hormone Signal Sequence- HaloTagExpressionMammalianMutationThe signal sequence from human growth hormone was…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
RA-Sec61β
Plasmid#153978PurposeDimerization dependent fluorescent protein RA domain fused to ER protein Sec61βDepositorInsertSec61 Transolocon Subunit Beta (SEC61B Human)
UseTagspAcGFP-C1ExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
V5-GFP-APEX2-NES_pLX208
Plasmid#202018Purposeexpresses GFP-APEX2 in the mammalian cytosol, lentiviral vectorDepositorInsertV5-GFP-APEX2-NES
UseLentiviralTagsGFP and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only