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Plasmid#172841PurposeMammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertDRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
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PGK-EGFP-FKBPF36V
Plasmid#199765Purposeplasmid for targeting dTAG into the AAVS1 locusDepositorInsertGFP-dTAG
UseTagsGFP and dTAGExpressionMammalian and WormMutationPromoterhPGKAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSHS212
Plasmid#87346PurposeCysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmidDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/C574SPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS7/myc-His
Plasmid#127488PurposeExpresses myc-tagged NDUFS7 in mammalian cellsDepositorInsertNDUFS7 (NDUFS7 Human)
UseTagsmyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-sRGECO
Plasmid#137125PurposeViral expression of sRGECODepositorHas ServiceAAV8InsertsRGECO
UseAAVTagsExpressionMutationE217DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-HA-ERK2 GOF
Plasmid#53200PurposeExpresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vectorDepositorInsertERK2 (MAPK1 Human)
UseTagsGST and HAExpressionBacterialMutationR67S/D321NPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-ChrimsonR-GFP
Plasmid#122063PurposeAAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.DepositorInsertChrimsonR-GFP
UseAAVTagsGFPExpressionMammalianMutationChrimson K176R mutantPromoterEF1aļ¼1.1kb short version)Available sinceMarch 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-Macro-Linker-eGFP
Plasmid#176067PurposeEGFP fused to the C-terminus of a Macrodomain & a hygromycin resistance cassetteDepositorInsertMacro
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
His SUMO TEV WT SPOP 28-359
Plasmid#194115PurposeWild type His-SUMO SPOP expression plasmidDepositorInsertSPOP (SPOP Human)
UseTags6His-SUMO-TEVExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330_VCL sgRNA / hSpCas9
Plasmid#172837PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-CRISPRoffv2.1-IRES-BlastR
Plasmid#207180PurposeLentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a PromoterDepositorInsertCRISPRoffv2.1
UseCRISPR and LentiviralTagsHA and TagBFPExpressionMammalianMutationPromoterEF1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-NLRP3
Plasmid#73955PurposeExpress EGFP-tagged human NLRP3 in mammalian cellsDepositorInsertNLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMV iEAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGGF000
Plasmid#48861PurposeEmpty entry vector for creating GreenGate plant resistance cassette modules.DepositorTypeEmpty backboneUseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV40-EF1a-hBCL10-SO
Plasmid#75256Purposeretroviral expression vector for expression of StrepOne-Tagged human BCL10DepositorInsertBcl10 (BCL10 Human)
UseRetroviralTagsStrepOne TagExpressionMammalianMutationPromoterpMSCV-LTRs, EF1aAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
DsRed-rab11 DN
Plasmid#12680DepositorInsertRAB11A (Homo sapiens) (RAB11A Human)
UseTagsDsRed and HAExpressionMammalianMutationchanged serine 25 to asparaginePromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAcUW51-gEgIL
Plasmid#11622DepositorInsertHSV-1 glycoprotein E and HSV-1 glycoprotein I
UseTagsHisExpressionInsectMutationDicistronic vector containing HSV-1 gE (KOS straiā¦PromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-WWE-Linker-eGFP
Plasmid#176072PurposeEGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassetteDepositorInsertWWE
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDHt/sk-CE
Plasmid#92128PurposeExpression of codon-optimized Cas9-eGFP fusion protein with Pcbh1 promoter in Trichoderma reeseiDepositorInsertCas9-eGFP
UseCRISPR; Trichoderma reesei expressionTagsEGFPExpressionMutationPromoterPcbh2Available sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-MCN-10His-mStayGold (E138D)
Plasmid#211361PurposeBacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.DepositorInsertmStayGold
UseTags10xHis-tagExpressionBacterialMutationE138DPromoterT7Available sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only