-
Plasmid#128177PurposeExpresses R. toruloides codon-optimized SpCas9 under PGK1 promoterDepositorInsertSpCas9-NLS3
UseCRISPRTagsExpressionYeastMutationPromoterpPGK1Available sinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL3-pegRNA-attL2
Plasmid#213052PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR3
Plasmid#213051PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMPRAv3:minP-GFP
Plasmid#109036PurposeMPRA v3 minimal promoter with GFP donorDepositorInsertemGFP
UseTagsExpressionMammalianMutationPromoterminPAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS3799-gRNA-Csy4-template
Plasmid#182827PurposeSpCas9 gRNA-Csy4 templateDepositorInsertSpCas9 gRNA scaffold + Csy4 sequence
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-iCre
Plasmid#89573PurposeExpresses iCre under pCAGDepositorInsertiCre
UseCre/Lox and Synthetic BiologyTagsNLSExpressionMammalianMutationPromoterAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWS5321-CRISPRai-Markerless
Plasmid#182828PurposeSaccharomyces cerevisiae inducible CRISPRai vector - markerlessDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMPRAv3:∆luc:∆xbaI
Plasmid#109035PurposeMPRA v3 empty backboneDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoternoneAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTPGI_dCas9_VP64
Plasmid#49013Purposeencodes yeast-optimized dCas9_VP64 synthetic transcription factorDepositorInsertdCas9_VP64 (codon-optimized for expression in S. cerevisiae)
UseCRISPR and Synthetic BiologyTagsSV40 NLSExpressionYeastMutationPromoterpTPGI (galactose+aTc inducible)Available sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWS5396-CRISPRai-KanR
Plasmid#182835PurposeSaccharomyces cerevisiae inducible CRISPRai vector - KanR markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUBC_V5_MS2-mCherry
Plasmid#68420PurposeTransient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoterDepositorInsertMS2
UseSynthetic BiologyTagsV5 and mCherryExpressionMammalianMutationMS2: V75E/A81GPromoterhuman UbCAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWS5398-CRISPRai-HygR
Plasmid#182837PurposeSaccharomyces cerevisiae inducible CRISPRai vector - HygR markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-nmdCas9-3XmCherry
Plasmid#64110PurposeExpression of Nm dCas9-3XmCherry in mammalian cellsDepositorInsertNm dCas9
UseCRISPR and LentiviralTags3XmCherryExpressionMammalianMutationPromoterCMV-TOAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWS5390-CRISPRai-URA3
Plasmid#182829PurposeSaccharomyces cerevisiae inducible CRISPRai vector - URA3 markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS5394-CRISPRai-MET17
Plasmid#182833PurposeSaccharomyces cerevisiae inducible CRISPRai vector - MET17 markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS5392-CRISPRai-HIS3
Plasmid#182831PurposeSaccharomyces cerevisiae inducible CRISPRai vector - HIS3 markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS5393-CRISPRai-TRP1
Plasmid#182832PurposeSaccharomyces cerevisiae inducible CRISPRai vector - TRP1 markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS5395-CRISPRai-LYS2
Plasmid#182834PurposeSaccharomyces cerevisiae inducible CRISPRai vector - LYS2 markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS5399-CRISPRai-ZeoR
Plasmid#182838PurposeSaccharomyces cerevisiae inducible CRISPRai vector - ZeoR markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS5391-CRISPRai-LEU2
Plasmid#182830PurposeSaccharomyces cerevisiae inducible CRISPRai vector - LEU2 markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only