Addgene

We narrowed to 684 results for: pcaggs

Showing: 401 - 420 of 684 results

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pCAG-T3-hCAS-pA

Plasmid

#48625

Purpose

Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Kunihiko Naito, Wataru Fujii

Article

Fujii et al Nucleic Acids Res. 2013 Aug 30.

Insert

Codon optimized Cas9

Use

CRISPR

Tags

FLAG, NLS, and polyA

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-mWasabi-G

Plasmid

#164161

Purpose

Expresses mWasabi under the control of CAG promoter, basic vector, multiclonig site at C terminal

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mWasabi

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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modified pcAT7-Glo1

Plasmid

#160996

Purpose

Splicing reporter backbone

Depositor

Brenton Graveley

Article

Adamson et al Genome Biol. 2018 Jun 1;19(1):71. doi: 1

Insert

hemoglobin subunit beta (HBB Human)

Use

Tags

Expression

Mammalian

Mutation

Repeated intron 1 with 40 nucelotide deletion

Promoter

CAG

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-NLGN3 R451C

Plasmid

#59319

Purpose

Expression plasmid of HA-tagged human Neuroligin 3 harboring an R451C mutation

Depositor

Peter Scheiffele

Article

Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

Insert

NLGN3 R451C (NLGN3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Arginine 451 to Cysteine (R451C mutation)

Promoter

CAG

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-NLGN4 D396*

Plasmid

#59321

Purpose

Expression plasmid of HA-tagged human Neuroligin 4 harboring a D396* nonsense mutation

Depositor

Peter Scheiffele

Article

Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

Insert

NLGN4 D396* (NLGN4X Human)

Use

Tags

HA

Expression

Mammalian

Mutation

D396* nonsense mutation, rest of ORF deleted

Promoter

CAG

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-hCasD10A-pA

Plasmid

#51638

Purpose

Expresses Cas9-nickase in mammalian cells and zygotes.

Depositor

Wataru Fujii

Article

Fujii et al Biochem Biophys Res Commun. 2014 Jan 31.

Insert

Codon-optimized Cas9 D10A

Use

CRISPR

Tags

FLAG, NLS, and polyA

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only
pBW808_pCAG-PV5-VloxP-loxN-F3-GFP-BGHpA

Plasmid

#87584

Purpose

PV5 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJK03_Rho_basal_DsRed

Plasmid

#173490

Purpose

Vector backbone used to clone MPRA libraries

Depositor

Barak Cohen

Article

White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

Insert

Bovine Rhodopsin promoter driving DsRed fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Bovine Rhodopsin

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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PSD95-TS:YSOG2

Plasmid

#42227

Depositor

Roger Tsien

Article

Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

Insert

PSD95

Use

Tags

TS:YSOG2

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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DAXX-myc

Plasmid

#1851

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

DAXX (Daxx Mouse)

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pBW829_pCAG-pExpr-LUT

Plasmid

#87554

Purpose

Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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PSD95-TS:YSOG1

Plasmid

#42226

Depositor

Roger Tsien

Article

Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

Insert

PSD95

Use

Tags

TS:YSOG1

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

Plasmid

#87560

Purpose

Expresses aa397-423 of Flp recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO aa397-423

Use

Cre/Lox and Synthetic Biology

Tags

PYL

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

Plasmid

#87559

Purpose

Expresses aa1-396 of Flp recombinase fused to ABI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO aa1-396

Use

Cre/Lox and Synthetic Biology

Tags

ABI

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pARC (pKD-G3)

Plasmid

#62682

Purpose

Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP123

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pATI (pKD-G7)

Plasmid

#62686

Purpose

Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP123/amiR-eGFP419

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBW811_pCAG-PV8-F14-GFP-T2A-mCherry-BGHpA

Plasmid

#87587

Purpose

PV8 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP, mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-HypaCAS9-pA

Plasmid

#131467

Purpose

Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.

Insert

Codon optimized HypaCas9

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBW1944_ hU6-PV4-loxP-BbsI-ENTRY-gRNA

Plasmid

#87572

Purpose

PV4 gRNA entry vector for CRISPR decoder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBW1943_ hU6-PV3-FRT-loxP-BbsI-ENTRY-gRNA

Plasmid

#89056

Purpose

PV3 gRNA entry vector for CRISPR decoder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge