Addgene

We narrowed to 10,628 results for: gan

Showing: 401 - 420 of 10628 results

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pMOD4 GFP

Plasmid

#19181

Depositor

Al Fisher

Article

Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

Insert

Green fluorescent protein

Use

Tags

FLAG and daf-12 N-term 328 amino acids

Expression

Worm

Mutation

S65C, contains synthetic introns for C. elegans

Promoter

Available since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pCZGY3196

Plasmid

#172696

Purpose

Prgef-1-gfp1-10 Expresses split GFP parts 1-10 in all C. elegans neurons (only shows active fluorescence when binds to GFP11)

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

Prgef-1

Use

Tags

GFP 1-10

Expression

Worm

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJA50

Plasmid

#59931

Purpose

gRNA for cleavage at unc-58(e665) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

unc-58(e665) gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pMLS546

Plasmid

#188883

Purpose

Integrating Pmex-5::Cas9 expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9

Use

Tags

Expression

Worm

Mutation

C. elegans codom optimization

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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B1979

Plasmid

#59181

Purpose

Expresses optimized blasticidin S resistance gene (BSD) for C. elegans

Depositor

Craig Mello

Article

Kim et al Genetics. 2014 May 30. pii: genetics.114

Insert

Blasticidin S resistance

Use

Tags

Expression

Worm

Mutation

Promoter

rpl-28

Available since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pOX + kqt3

Plasmid

#16138

Depositor

Larry Salkoff

Article

Wei et al J Biol Chem. 2005 Jun 3. 280(22):21337-4

Insert

Caenorhabditis elegans potassium channel (kqt-3 Nematode)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

lacks aa1-16 compared to GenBank reference AY5729…

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pCZGY405

Plasmid

#172694

Purpose

Punc-25-mcherry::rab-3 Expresses Red-tagged synapses specifically in C. elegans GABAergic motor neurons

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

rab-3 (rab-3 Nematode)

Use

Tags

Punc-25 and mcherry

Expression

Worm

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJA55

Plasmid

#59935

Purpose

gRNA for cleavage near sqt-1(e1350) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

sqt-1(e1350) gRNA2

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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dg264_pENTR_ChronosCel

Plasmid

#66102

Purpose

Entry clone containing Chronos coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

Depositor

Dominique Glauser

Article

Schild et al Genetics. 2015 May 28. pii: genetics.115

Insert

Chronos

Use

Gateway

Tags

Expression

Mutation

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pBN40

Plasmid

#86715

Purpose

C. elegans injection marker

Depositor

Peter Askjaer

Article

Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

Insert

Plmn-1::gfp::his-58

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJA14

Plasmid

#59929

Purpose

gRNA for cleavage at rde-1(H974) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rde-1(H974) gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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GSPD1_R252L

Plasmid

#224153

Purpose

IPTG-inducible expression of C. elegans protein GSPD-1(R252L) with a His tag

Depositor

Katherine Walstrom

Article

Loges et al MicroPubl Biol. 2021 Sep 10;2021:10.1791

Insert

GSPD-1(R252L)

Use

Tags

LeuGlu-6xHis

Expression

Bacterial

Mutation

R252L

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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GSPD1_AJ

Plasmid

#224152

Purpose

IPTG-inducible expression of C. elegans protein GSPD-1 with a His tag

Depositor

Katherine Walstrom

Article

Loges et al MicroPubl Biol. 2021 Sep 10;2021:10.1791

Insert

GSPD-1 (B0035.5 Nematode)

Use

Tags

LeuGlu-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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GSPD1_D60N

Plasmid

#224154

Purpose

IPTG-inducible expression of C. elegans protein GSPD-1(D60N) with a His tag

Depositor

Katherine Walstrom

Article

Loges et al MicroPubl Biol. 2021 Sep 10;2021:10.1791

Insert

GSPD-1(D60N)

Use

Tags

LeuGlu-6xHis

Expression

Bacterial

Mutation

D60N

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pHIT179

Plasmid

#204510

Purpose

Bacterial expression of CSR-1a with 6xHis and maltose binding protein tags for in vitro assays

Depositor

Yuji Kohara

Article

Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.

Insert

csr-1a (C. elegans) (csr-1 Nematode)

Use

Tags

6xHis, MBP

Expression

Bacterial

Mutation

Promoter

T7lac

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGC461

Plasmid

#71363

Purpose

distal tip cell expression of DAF-16 in C. elegans

Depositor

Jane Hubbard

Article

Michaelson et al Development. 2010 Feb;137(4):671-80. doi

Insert

daf-16 (daf-16 Nematode)

Use

Tags

GFP(65C)

Expression

Worm

Mutation

Promoter

Blelloch et al., 1999; Mathies et al., 2003

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJA46

Plasmid

#59928

Purpose

gRNA for cleavage at rde-1(D801) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rde-1(D801) gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pJA45

Plasmid

#59927

Purpose

gRNA for cleavage at rde-1(D718) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rde-1(D718) gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Wt1

Plasmid

#41082

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Wt1 (-KTS alternative isoform) (Wt1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetO + minimal CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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mCRISPRed pRSETB

Plasmid

#159454

Purpose

Expresses the pH-resistant long Stokes shift red fluorescent protein mCRISPRed

Depositor

Oliver Griesbeck

Article

Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

Insert

mCRISPRed

Use

Tags

10 x His-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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