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We narrowed to 21,291 results for: crispr

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pFS_0390_pET30-RT-CRISPR_Eubacterium_saburreum_DSM_3986_ARRAY_1_RC

Plasmid

#117002

Purpose

Expresses EsRT-Cas1-Cas2 under pT7lac promoter, encodes EsCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Eubacterium saburreum RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0389_pET30-RT-CRISPR_Eubacterium_saburreum_DSM_3986_ARRAY_1

Plasmid

#117001

Purpose

Expresses EsRT-Cas1-Cas2 under pT7lac promoter, encodes EsCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Eubacterium saburreum RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0374_pET30-RT-CRISPR_Teredinibacter_turnerae_T8412_ARRAY_1_RC

Plasmid

#116986

Purpose

Expresses TtRT-Cas1-Cas2 under pT7lac promoter, encodes TtCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Teredinibacter turnerae T8412 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0372_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_2_RC

Plasmid

#116984

Purpose

Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Azospirillum lipoferum 4B RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0373_pET30-RT-CRISPR_Teredinibacter_turnerae_T8412_ARRAY_1

Plasmid

#116985

Purpose

Expresses TtRT-Cas1-Cas2 under pT7lac promoter, encodes TtCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Teredinibacter turnerae T8412 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0370_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_1_RC

Plasmid

#116982

Purpose

Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Azospirillum lipoferum 4B RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0371_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_2

Plasmid

#116983

Purpose

Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Azospirillum lipoferum 4B RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgAMDHD1

Plasmid

#102316

Purpose

genetic depletion of AMDHD1

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgAMDHD1 (AMDHD1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR sgNFS1

Plasmid

#102979

Purpose

Cutting NFS1 genomic locus

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

sgNFS1 (NFS1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgC17orf89-2

Plasmid

#86135

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting C17orf89

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA targeting C17orf89 (NDUFAF8 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgC17orf89-3

Plasmid

#86136

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting C17orf89

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA 3 targeting C17orf89 (NDUFAF8 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgC17orf89-1

Plasmid

#86137

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting C17orf89

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA 1 targeting C17orf89 (NDUFAF8 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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TU#1805_CRISPR_unc-73_exon2

Plasmid

#82359

Purpose

to create unc-73B null allele

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Cas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PDi-CRISPRn

Plasmid

#73500

Purpose

Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

Cas9
rtTA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

CAG and TRE

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-multi-CRISPR

Plasmid

#85402

Purpose

Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression

Depositor

Qin Yan

Article

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pNOC-ARS-CRISPR-v2

Plasmid

#99863

Purpose

Expresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker.

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

Expression

Mutation

Promoter

Ribi

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NC-CRISPRi (Gen3)

Plasmid

#73499

Purpose

Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

dCas9-KRAB

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - EGFP sgRNA 1

Plasmid

#51760

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

Feng Zhang

Article

Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

Inserts

Cas9
Puromycin resistance
EGFP sgRNA 1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS and U6

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgLKB1 clone2

Plasmid

#162126

Purpose

Lentiviral sgRNA plasmid targeting human LKB1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgLKB1 (STK11 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPR-RFP entry

Plasmid

#192278

Purpose

Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

RFP flanked by type V-M CRISPR repeat sequences

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PJ23119

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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