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Plasmid#160508PurposeExpresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applicationsDepositorInsertIntCCCre
UseCre/Lox and LentiviralTagsmCeruleanExpressionMammalianMutationPromoterCAGAvailable sinceJan. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-Avi-Cerulean-RanGAP
Plasmid#79881PurposeUbiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
UseTagsAviExpressionBacterialMutationPromoterUbiquitin promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXL-CAG-ICeuI-Cerulean-2A-sHRPbNLG1
Plasmid#79912PurposepiggyBac transposon vector, Cerulean-2A-sHRPbNLG1DepositorInsertCerulean-2A-sHRPbNLG1
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2 Krt4 Src (YF)-uniRapR-cerulean
Plasmid#45509DepositorInsertSrc (YF)-uniRapR-cerulean
UseZebrafish expressionTagsceruleanExpressionMutationY732FPromoterkrt4Available sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
PhOTO-C (pMTB:cyto-Dendra2-2A-H2B-Cerulean)
Plasmid#92402PurposeConstitutive expression of cytosolic green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.DepositorInsertsDendra2
Cerulean
UseZebrafish expressionTagsH2B tag and No tag, cytosolic expressionExpressionMutationPromoterbactin2Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cerulean(159-238) in pcDNAI/Amp
Plasmid#60898PurposeThis is a vector that attaches Cerulean(159-238) to the N-terminus of a protein.DepositorInsertCerulean(159-238)
UseTagsExpressionMammalianMutationContains N164H substitutionPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME-mCerulean3-TUBB1A (JDW 1367)
Plasmid#224503PurposeGateway compatible middle entry clone containing mCerulean3 fused to TUBB1A for visualizing tubulin in cellsDepositorInsertmCerulean3-TUBB1A
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME H2B mCerulean (JDW 1150)
Plasmid#224543PurposeA Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleusDepositorInsertH2B-mCerulean
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
MsACR1-TS-Kv-P2A-mCerulean3-pAAV-CaMKII
Plasmid#204970PurposeSoma-targeted neuronal expression of MsACR1 and mCerulean3 separated by a P2A site.DepositorInsertMsACR1
UseAAVTagsmCerulean3ExpressionMutationPromoterCaMKIIAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC-DEST-cjSTELLA-mCerulean-PuroTK
Plasmid#141029PurposeReporter integrationDepositorInsertmCerulean
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cerulean(1-158)Stop in pcDNA1/amp
Plasmid#60894PurposeThis vector attaches mCerulean(1-158) to the C-terminus of a protein.DepositorInsertCerulean(1-158)Stop
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cerulean-Epac2(R53A)-Venus
Plasmid#138197PurposeFRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.DepositorInsertCerulean-Epac2(R53A)-Venus (Rapgef4 Mouse, Synthetic)
UseTagsCerulean and VenusExpressionMammalianMutationContains arginine-to-alanine substitution at resi…PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cerulean-Epac2(R466A)-Venus
Plasmid#138199PurposeFRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.DepositorInsertCerulean-Epac2(R466A)-Venus (Rapgef4 Mouse, Synthetic)
UseTagsCerulean and VenusExpressionMammalianMutationContains arginine-to-alanine substitution at resi…PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cerulean-Epac2(L426W)-Venus
Plasmid#138200PurposeFRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.DepositorInsertCerulean-Epac2(L426W)-Venus (Rapgef4 Mouse, Synthetic)
UseTagsCerulean and VenusExpressionMammalianMutationContains leucine-to-tryptophan substitution at re…PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry
Plasmid#34911DepositorInsertpresynaptic mGRASP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-mCerulean3-P2A-mKOFP2-CAAX
Plasmid#75154PurposeNuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.DepositorInsertH2B-mCerulean3-P2A-mKOFP2-CAAX
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mCerulean 156-239-GGGS-TDP-43
Plasmid#162617PurposeAllows for transcription of mCerulean fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assaysDepositorInsertTARDBP (TARDBP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus
Plasmid#105805PurposeConcomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.DepositorTypeEmpty backboneUseFlp-in competentTagsORF1: 3C-Cerulean; ORF2: TEV-VenusExpressionMammalianMutationPromoterAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX
Plasmid#75177PurposeNuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.DepositorInsertH2B-mCerulean3-P2A-mKOFP2-CAAX
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
α-actinin-4-M522-sstFRET(Venus/Cerulean) in pUNO1
Plasmid#190799PurposeImaging of force-sensitive α-actinin-4 in live cellsDepositorInsertα-actinin-4-Venus-Cerulean-522 (ACTN4 Human, Synthetic)
UseTagscerulean and venusExpressionMammalianMutationPromoterhEF1-HTLVAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only