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Plasmid#74914PurposeMammalian expression of mNanog (Y42E, K43T)DepositorInsertNanog (Nanog Mouse)
UseTagsExpressionMammalianMutationK43T of Nanog protein and Y42E of Nanog homeodoma…PromoterCAGAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
LZRS ATG12 ires zeo
Plasmid#67531PurposeFor retroviral based stable expression of human ATG12DepositorInsertAPG12 (ATG12 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
mADAM8 deltaMP in pQCXIP cFLAG
Plasmid#19149DepositorInsertADAM8 (Adam8 Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationdelta 228-397PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEF-TAZ-N-Flag (-4)
Plasmid#40914DepositorInsertTAZ (Wwtr1 Mouse)
UseTagsFlagExpressionMammalianMutationVector has no SV40 origin, and lacks the final fo…PromoterAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
hTln1-R1R2
Plasmid#191440PurposeExpresses the human TLN1 R1R2 domains in bacteriaDepositorInsertHuman Talin1 R1R2 domains (TLN1 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-del72-136-pEGFP-C3
Plasmid#191411PurposeTransfection of this plasmid will express "VBik-d72-136" (Venus fused to the N-terminus of the BH3-only protein, Bik that has amino acids 72-136 deleted).DepositorInsertBcl-2-interacting killer (BIK Human)
UseTagsVenusExpressionMammalianMutationwith deletion of amino acids 72-136 (del72-136) i…PromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pLV-SARS-CoV-2 5’UTR ΔSL1-Luciferase
Plasmid#191481PurposeLuciferase reporter for SARS-CoV-2 5' UTR ΔSL1DepositorInsertSARS-CoV-2 5' UTR ΔSL1
UseLentiviral and LuciferaseTagsExpressionMutationMissing the stem-loop 1 region of SARS-CoV-2 5…PromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACE-K2P4.1-StrepTagII
Plasmid#191474PurposeExpresses K2P4.1 with C-terminal StrepTagIIDepositorInsertK2P4.1 (KCNK4 Human)
UseTagsExpressionInsectMutationN104Q N108QPromoterpolHAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKSB-AeU6-3gRNA
Plasmid#183914PurposegRNA-expressing plasmid under Aedres aegypti AAEL017763 U6 promoterDepositorInsertgRNA scaffold for protospacer cloning into BbsI sites
UseTagsExpressionMutationPromoterAedes aegypti U6 promoter (AAEL017763)Available sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-SQSfl
Plasmid#171010PurposeHiLITR protease with full-length SQS targeting information (ER)DepositorInsertEGFP-uTEV1-SQS(FL)
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Halo-Tev- HSPB7(zebrafish) C49S
Plasmid#108914PurposeExpress C49S mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cellsDepositorInsertHalo-TEV-HSPB7
UseTagsHalo-TEVExpressionMammalianMutationCysteine 49 to SerinePromoterSP6Available sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
A3H HapII R175/6E-Cas9n-UGI
Plasmid#119142PurposeBase editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EEDepositorInsertAPOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI
UseCRISPRTagsExpressionMutationA3H Hap II RR175/6EEPromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SpCas9-GFP-U6-gRNA
Plasmid#79144PurposeAll-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSCDepositorInsertSpCas9
UseCRISPRTags2A-GFPExpressionMammalianMutationPromoterCAGAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
BLADE-182
Plasmid#134914PurposesgRNA targeting GFP to be used in nanoblade systemDepositorInsertGFP
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterU6Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Pard3-EGFP-PIF6
Plasmid#154914PurposeFull length zebrafish Pard3 (Gene ID: 403050), tagged with EGFP fluorescent protein and Arabidopsis PIF6 (as in construct Addgene ID 154913).DepositorInsertpar-3 family cell polarity regulator alpha, b (pard3ab Zebrafish)
UseXenopus/avian/zebrafishTags1-100 amino acids of Arabidopsis PIF6 and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1328 - pAAV EF1a HA-hM3D(Gq)
Plasmid#89149PurposeAn AAV packaging plasmid that expresses HA-tagged hM3D(Gq) excitatory DREADD from the EF1a promoter.DepositorInsertHA-tagged hM3D(Gq)
UseAAVTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX304-YAP1 _60-89
Plasmid#59144PurposeYAP1 mutants that harbored a deletion of amino acide at 60-89 position, which disrupts binding to TEAD transcription factorsDepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationaa60-89 deletedPromoterCMVAvailable sinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACE-K2P10.1-StrepTagII
Plasmid#191475PurposeExpresses K2P10.1 with C-terminal StrepTagIIDepositorUseTagsExpressionInsectMutationN144Q (uniprot P57789-1)PromoterpolHAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
SnapTag-Binder
Plasmid#179142PurposeBinder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).DepositorInsertSspB
UseTagsSNAP-tagExpressionMammalianMutationY11K/A15E, residue numbering from pdb: 1ou9PromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only