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Plasmid#75370PurposeExpresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherryDepositorInsertCRY2 residues 1-498 (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationW349RPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEO-CaMKII-TVA-P2A-N2cG
Plasmid#172363PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from excitatory cortical neurons which do not express cre-recombinaseDepositorInsertTVA + B19-N2cG
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-fDIO-Cre (AAV1)
Viral Prep#121675-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)
Plasmid#85008PurposeFor Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.DepositorInsertEGFP-ires-tTA
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgRNA.SFFV.tRFP
Plasmid#169941PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tRFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-lck-GCaMP6f (AAV5)
Viral Prep#52924-AAV5PurposeReady-to-use AAV5 particles produced from pZac2.1 gfaABC1D-lck-GCaMP6f (#52924). In addition to the viral particles, you will also receive purified pZac2.1 gfaABC1D-lck-GCaMP6f plasmid DNA. gfaABC1D-driven (similar to GFAP promoter) lck-GCaMP6f calcium sensor expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsNoneAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGOAL19
Plasmid#20190DepositorTypeEmpty backboneUseMarker cassetteTagsExpressionMutationPromoterAvailable sinceMay 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pOPING
Plasmid#26046DepositorInsertSS peptide-LacZ-KHHHHHH
UseTagsKHHHHHH and MGILPSPGMPALLSLVSLLSVLLMGCVAETGExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJR019
Plasmid#198834PurposeInactivation of G protein subunits through Pertussis toxin (PTX)DepositorInsert15xUAS::PTX-SL2-GFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2F
Plasmid#15800DepositorInsertUbiquitin conjugating enzyme UbE2F (UBE2F Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
Halo-Tev-Flag-Ube2v2
Plasmid#110070PurposeExpress Halo-Tev-Flag-Ube2v2 (human) in mammalian cellsDepositorInsertHalo-Tev-Flag-Ube2v2 (UBE2V2 Human)
UseTagsHalo-TEV-FlagExpressionMammalianMutationPromoterT7Available sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-mCherry (AAV5)
Viral Prep#114469-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTrex-Luc-Neo
Plasmid#48336PurposeTrasgenically expresses firefly luciferase in T. cruzi with neomycin selectionDepositorInsertfirely luciferase
UseTrypanasoma cruzi expressionTagsExpressionMutationPromoterT. cruzi rRNAAvailable sinceJuly 20, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-Amp_CRISPIE donor B2
Plasmid#172843PurposeCRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mRuby3
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-TRAF1
Plasmid#111199Purposefluorescent fusion proteinDepositorInsertTRAF1 (TRAF1 Human)
UseTagsEGFP-FLAGExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUB6-CDC34-HA (pRKB261)
Plasmid#99145PurposeMammalian expression construct to express HA-tagged CDC34DepositorInsertCDC34 (CDC34 Human)
UseTagsHAExpressionMammalianMutationPromoterpTightAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28CLY1
Plasmid#21760DepositorInsertECFP -EYFP linked by flexible one SGGSGG repeats
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 pegRNA2 with trimmed attP
Plasmid#222347PurposepegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installationDepositorInsertAAVS1 attP-installation pegRNA2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf5(WT)-HA
Plasmid#79427PurposeExpresses C-terminally HA-tagged Arf5(WT) in mammalian cellsDepositorInsertArf5 (ARF5 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_hSyn_NES-Caprola_off-mEGFP_WRPE-SV40
Plasmid#194692PurposehSyn1 driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through AAV transductionDepositorInsertCaprola_off-mEGFP
UseAAVTagsmEGFPExpressionMutationPromoterhSyn1Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only