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Plasmid#108723PurposeExpresses N-terminus to aa229 of Cre recombinase fused to GID1 CIDDepositorInsertCre recombinase fragment N-terminus to aa229
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2229_pCAG-PV1-GAI-L1-iCre-230C-NLS-BGHpA
Plasmid#108724PurposeExpresses aa230 to C-terminus of Cre recombinase fused to GAI CIDDepositorInsertCre recombinase fragment aa230 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.26 EC10-Fc-His
Plasmid#72189PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertDscam1_7.27.26
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.23 EC10-Fc-His
Plasmid#72187PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertDscam1_7.27.23
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.26 EC10-AP-His
Plasmid#72063PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertDscam1_7.27.26
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN-mPAGFP
Plasmid#114114PurposeMammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry NEDD4 WW2 neg ctrl
Plasmid#112030PurposeMode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide)DepositorInsertNEDD4 WW2 domain fused to C-terminal split mCherry, neg ctrl interacting peptide fused to N-terminal split mCherry
UseTagsSplit mCherryExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEMS2235
Plasmid#111888PurposeHigh copy number plasmid containing Ple320 (POU4F1 MiniPromoter) insert.DepositorInsertPle320 (POU4F1 Human)
UseUnspecifiedTagsExpressionMutationPromoterPle320 (POU4F1 MiniPromoter)Available sinceAug. 10, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS2236
Plasmid#111889PurposeHigh copy number plasmid containing Ple321 (TUBB3 MiniPromoter) insert.DepositorInsertPle321 (TUBB3 Human)
UseUnspecifiedTagsExpressionMutationPromoterPle321 (TUBB3 MiniPromoter)Available sinceAug. 10, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX6P-1 PUM FL
Plasmid#112997PurposeFor protein expression and purification of Drosophila pumilio (amino acids 566-1090)DepositorInsertpumilio (pum Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSM4
Plasmid#101642PurposeEncodes the fluorescent protein KCyG4219 for constitutive expression in E. coli.DepositorInsertKCyG4219
UseTags6XHisExpressionBacterialMutationC-terminal residues (221-223) deletion. Ser220Val…PromoterAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
KCC4_HUMAN_D0
Plasmid#79744PurposeThis plasmid encodes the kinase domain of KCC4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKCC4 (SLC12A7 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KS6A1_HUMAN_D1
Plasmid#79720PurposeThis plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKS6A1 (RPS6KA1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK10_HUMAN_D0
Plasmid#79722PurposeThis plasmid encodes the kinase domain of STK10. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertSTK10 (STK10 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS2023
Plasmid#49130PurposeAAV plasmid with hs1218 promoter driving expression of iCre.DepositorInsertssAAV-hs1218-iCre
UseAAVTagsExpressionMammalianMutationPromoterhs1218Available sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS2024
Plasmid#49131PurposeAAV plasmid with hs671 promoter driving expression of iCre.DepositorInsertssAAV-hs671-iCre
UseAAVTagsExpressionMammalianMutationPromoterhs671Available sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS2025
Plasmid#49132PurposeAAV plasmid with NOV (Ple303) promoter driving expression of iCre.DepositorInsertssAAV-Ple303-iCre
UseAAVTagsExpressionMammalianMutationPromoterNOVAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS2050
Plasmid#49135PurposeAAV plasmid with CAGGS promoter driving expression of EmGFP.DepositorInsertssAAV-CAGGS-emGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
Plxnb1(S)-Fc-His
Plasmid#72127PurposeExpresses the N-terminal extracellular region of the PlexinB1 protein following proteolytic cleavage (ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertPlxnb1 (Plxnb1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plxnb2(S)-AP-His
Plasmid#72003PurposeExpresses the N-terminal extracellular region of the PlexinB2 protein following proteolytic cleavage (ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertPlxnb2 (Plxnb2 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only