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Addgene

pEMS2236
(Plasmid #111889)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 111889 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    Unknown
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ple321
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1829
  • Entrez Gene
    TUBB3 (a.k.a. CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4)
  • Promoter Ple321 (TUBB3 MiniPromoter)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid commercially produced by Genscript . Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid, including the MiniPromoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS2236 was a gift from Elizabeth Simpson (Addgene plasmid # 111889 ; http://n2t.net/addgene:111889 ; RRID:Addgene_111889)
  • For your References section:

    New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression in Retinal Ganglion Cells of Mouse and Primate Retina. Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW. Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118. 10.1089/hum.2018.118 PubMed 30062914