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Plasmid#228722PurposeExpresses N-terminal ABE8e-Nme2Smu nCas9 (D16A) base editor with E932R mutation in mammalian cellsDepositorInsertN-terminal ABE8e with Nme2Smu nCas9 (D16A) E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2702-CMV-iNme2-ABE8e-nt
Plasmid#228723PurposeExpresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cellsDepositorInsertN-terminal ABE8e with iNme2 nCas9 (D16A)
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A D844G E868K K870R D873A D911G K929…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2700-CMV-Nme2-ABE8e-nt-E932R
Plasmid#228721PurposeExpresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cellsDepositorInsertN-terminal ABE8e with Nme2 nCas9 (D16A) E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2712-CMV-eNme2-C-ABE8e-i1-L10
Plasmid#228733PurposeExpresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cellsDepositorInsertDomain inlaid eNme-C nCas9 (D16A)-i1-L10
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-deltaCKAR
Plasmid#222430PurposeFRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.DepositorInsertNucleus delta-selective C kinase activity reporter
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
T2A GFP H2B
Plasmid#227722PurposePlasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp ResistantDepositorInsert(nlp-51 homology) T2A 3xNLS GFP (nlp-51 Nematode)
UseCloning vectorTagsT2A 3xNLS GFPExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
T2A 3xNLS GFP
Plasmid#227721PurposePlasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp ResistantDepositorInsertT2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Sc++
Plasmid#198545PurposeExpresses human codon-optimized Sc++ and blasticidin resistance: EFS promoter-Sc++-NLS-FLAG-P2A-BSDDepositorInsertSc++
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpG
Plasmid#198543PurposeExpresses human codon-optimized SpG and blasticidin resistance: EFS promoter-SpG-NLS-FLAG-P2A-BSDDepositorInsertSpG
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Golgi-deltaCKAR
Plasmid#222428PurposeFRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.DepositorInsertGolgi delta-selective C kinase activity reporter
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-cdl
Plasmid#207649PurposeOverexpression of heterologous L-cysteine desulfidase in E. coli MG1655DepositorInsertcdl
UseTags6xHisExpressionBacterialMutationPromoterlacUV5Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-cdl
Plasmid#207652PurposeOverexpression of heterologous L-cysteine desulfidase in E. coli MG1655DepositorInsertcdl
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDLterm
Plasmid#211903PurposeBase plasmid for dual-luciferase constructs to measure terminator strengthDepositorTypeEmpty backboneUseLuciferaseTagsExpressionPlantMutationPromoter35S enhancer + 35S minimal promoterAvailable sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSDM052
Plasmid#194341PurposepUC mini-Tn7 vector expressing LseCas13aDepositorInsertCas13a(Lse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-U6.3-sgRNA-non-targeting-control -GFP
Plasmid#221844PurposeTol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoterDepositorInsertsEGFP
non-targeting control sgRNA- GCACTGCTACGATCTACACC
UseCRISPR; Tol2 transposon optimised for chick expre…TagsExpressionMammalianMutationPromoterGACG and U6.3 chickAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTP489
Plasmid#216366PurposexNup155-Nb1t (+3Cys) Nanobody productionDepositorInsertxNup155-Nb1t (+3Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC263
Plasmid#216367PurposexhNup155-Nb2i Nanobody productionDepositorInsertxhNup155-Nb2i
UseAffinity Reagent/ AntibodyTags14xHis-Avi-SUMOEu1ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC261
Plasmid#216371PurposexhNup155-Nb3i Nanobody productionDepositorInsertxhNup155-Nb3i
UseAffinity Reagent/ AntibodyTags14xHis-Avi-SUMOEu1ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTP728
Plasmid#216374PurposexNup358-Nb1t (+3Cys) Nanobody productionDepositorInsertxNup358-Nb1t (+3Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC226
Plasmid#216375PurposexhNup358-Nb2t (+3Cys) Nanobody productionDepositorInsertxhNup358-Nb2t (+3Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only