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We narrowed to 11,160 results for: 110

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pHsp70(K71E)-GFP

Plasmid

#228196

Purpose

expressed human Hsp70 (K71E mutation) with a GFP fluorescent protein tag

Depositor

Stephen Benkovic

Article

French et al Proc Natl Acad Sci U S A. 2013 Feb 12;11

Insert

HSP70(K71E) (HSPA1A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

K71E

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLIX401 APE1 WT

Plasmid

#227190

Purpose

Inducible lentiviral expression of WT APE1

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

apurinic/apyrimidinic endonuclease APE1 (APEX1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLIX401 APE1 SO

Plasmid

#227192

Purpose

Inducible lentiviral expression of SO (Slow Off) APE1

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

apurinic/apyrimidinic endonuclease APE1 (APEX1 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

D283A D308A

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2D-A

Plasmid

#221734

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D683A in active site

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2Y-F

Plasmid

#221732

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Y680F in active site

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2D-E

Plasmid

#221731

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E in active site

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2

Plasmid

#221730

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-hsAgo2

Plasmid

#221718

Purpose

Expression of human Ago2 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2 (AGO2 Human)

Use

Fish expression

Tags

Expression

Mutation

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2

Plasmid

#221712

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TRBP-L326A/V336A/H338A/Y358A

Plasmid

#215895

Purpose

Expression of TRBP-L326A/V336A/H338A/Y358A

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

TRBP-L326A/V336A/H338A/Y358A (TARBP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRV1 CTFdeltaTA-FLAG

Plasmid

#217743

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRV1 (ADGRV1 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-5896

Promoter

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRV1 CTF-FLAG

Plasmid

#217710

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRV1 (ADGRV1 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-5890

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[V458A][Q459A][V460A][G461A]-FLAG

Plasmid

#199328

Purpose

mutated RHIM motive (interaction inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed V458, Q459,V460 and G461 to alanine

Promoter

Available since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199D]-FLAG

Plasmid

#199315

Purpose

mutated phosphorylation site (kinase acitivity permanent active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 to Aspartic Acid

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199A]-FLAG

Plasmid

#199313

Purpose

mutated phosphorylation site (kinase acitivity inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 to Alanine

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTP-PCR C3

Plasmid

#189620

Purpose

Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

Depositor

Bogumil Karas

Article

Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

Insert

Reduced Mitochondrial Genome of Thalassiosira pseudonana

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

Removed repeat region, 10-kbp deletion, and minor…

Promoter

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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tet pLKO.1-shCLTC v2 puro

Plasmid

#192348

Purpose

Lentiviral expression vector for an inducible shCLTC v2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

shCLTC v2 (CLTC Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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tet pLKO.1-shCLTC v1 puro

Plasmid

#192347

Purpose

Lentiviral expression vector for an inducible shCLTC v1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

shCLTC v1 (CLTC Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-KPNA1 zeo

Plasmid

#192329

Purpose

Lentiviral expression vector for an inducible 3xFLAG-KPNA1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KPNA1 (KPNA1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-BCAS2 zeo

Plasmid

#192319

Purpose

Lentiviral expression vector for an inducible 3xFLAG-BCAS2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BCAS2 (BCAS2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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