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Plasmid#140985PurposeEncodes a G alpha subunit (GNA12) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlpha12-RLuc8 (GNA12 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-Akt-STOPS
Plasmid#175009PurposePlasma membrane-targeted Akt-STOPS.DepositorInsertLyn-mCherry-Akt-STOPS
UseTagsN-terminal targeting sequence from Lyn kinase and…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoR-ATP
Plasmid#193820PurposeCMV overexpression of ChemR-ATP in mammalian cell linesDepositorInsertChemoR-ATP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-Akt1-aa1-149
Plasmid#73410PurposeExpresses HA tagged aa1-149 of AKT1DepositorInsertAKT1 (AKT1 Human)
UseTagsHA tagExpressionMammalianMutationExpresses aa 1-149 only of AKT1PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-2A-eGFP(W58X) pcDNA3.1(-)
Plasmid#172229PurposeExpresses an mCherry-2A-eGFP dual reporter that contains a UAG codon in position 58 (W58X) of eGFP, in mammalian cellsDepositorInsertmCherry-2A-eGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MYO10-HMM-Nanotrap
Plasmid#87255PurposeExpression plasmid to perform NanoSPD 2.0 assays in mammalian cells.DepositorInsertMYO10 (Myo10 Mouse)
UseTagsGFP-nanotrapExpressionMammalianMutationMyo10 is truncated to contain aa.1-941PromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO GFP hBub1 WT
Plasmid#59812PurposeAllows the integration of GFP hBub1 in the genome and Tet-inducible expression.DepositorInsertBub1 (BUB1 Human)
UseTagsEmGFPExpressionMammalianMutationPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Tsapphire-StagRFP-SrcAR
Plasmid#173018PurposeLong-Stokes-shift green-red FRET-based Src activity reporter for multiplexed imaging applications.DepositorInsertTsapphire-StagRFP-SrcAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1(-) mouse Egr3
Plasmid#107998PurposeExpresses mouse Egr3 in mammalian cellsDepositorInsertEgr3 (Egr3 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgO-sfGFP (Merlin)
Plasmid#136452PurposeMammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFPDepositorInsertglycoprotein O (UL74 Human betaherpesvirus 5)
UseTagsGly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-Akt1-aa120-433
Plasmid#73411PurposeExpresses HA tagged aa120-433 AKT1DepositorInsertAKT1 (AKT1 Human)
UseTagsHA tagExpressionMammalianMutationExpresses aa 120-433 of AKT1PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AU1-mTOR-L1460P
Plasmid#19995DepositorInsertmammalian target of rapamycin-L1460P (Mtor Rat)
UseTagsAU1ExpressionMammalianMutationChanged Leucine 1460 to ProlinePromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 CDK9 (D167N) HA (P#811)
Plasmid#14641DepositorInsertcdk9 D167N (CDK9 Human)
UseTagsHAExpressionMammalianMutationD167N. Kinase negative mutant. Please see deposit…PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
integrin alpha9 pcDNA1 neo
Plasmid#13585DepositorInsertintegrin alpha 9 (ITGA9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HNF1A-WT-Flag-UTR
Plasmid#183237PurposeExpression of FLAG tagged HNF1ADepositorInsertHNF1 homeobox A (HNF1A Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAJB8
Plasmid#19504DepositorInsertDNAJB8 (DNAJB8 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-emerin-EGFP-polyA
Plasmid#59747PurposeExpress emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertemerin-EGFP
UseTagsEGFPExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
Blue opsin mcherry ---- pcDNA3.1
Plasmid#62932PurposeBlue opsin tagged with mcherry on the C terminusDepositorInsertblue opsin
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-cyto-KB1691-Nluc
Plasmid#204326PurposeGPCR/G protein BRET biosensorDepositorInsertcytosolic KB1691-Nluc
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Ikkb-FLAG(K44A)
Plasmid#23299DepositorInsertIkkbeta (IKBKB Human)
UseTagsFlagExpressionMammalianMutationK44APromoterAvailable sinceApril 16, 2010AvailabilityAcademic Institutions and Nonprofits only