Skip to main content
Addgene
Showing: 4101 - 4120 of 14719 results

  1. Prp2-pRSETA

    Plasmid
    #61042
    Purpose
    Encodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag.
    Depositor
    Nils Walter
    Article
    Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145
    Insert
    Prp2
    Use
    Tags
    Histidine Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pNB754

    Plasmid
    #60157
    Purpose
    Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by LYS9 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    GrLuc
    Use
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Mutation
    Promoter
    LYS9pr
    Available since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60157-plasmid-map-sequence-id-96349

    Enlarge

  6. pNB691

    Plasmid
    #60135
    Purpose
    Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    RdLuc
    Use
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Mutation
    Promoter
    MET17pr
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60135-plasmid-map-sequence-id-96202

    Enlarge

  7. pNB753

    Plasmid
    #60148
    Purpose
    Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    GrLuc
    Use
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Mutation
    Promoter
    LEU1pr
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60148-plasmid-map-sequence-id-96305

    Enlarge

  16. pCAGGS-PHOSPHO2-TEV-Twin-Strep

    Plasmid
    #222016
    Purpose
    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO2 (PHOSPHO2 Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted stop codon (TAA)
    Promoter
    chicken β-actin promoter
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222016-plasmid-map-sequence-id-457428

    Enlarge

  17. pGEX-PHOSPHO2-TEV-Twin-Strep

    Plasmid
    #222017
    Purpose
    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in E. coli
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO2 (PHOSPHO2 Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Bacterial
    Mutation
    deleted stop codon (TAA)
    Promoter
    lac promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    222017-plasmid-map-sequence-id-457152

    Enlarge

Showing: 4101 - 4120 of 14719 results