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Plasmid#171717PurposeLuminal ER expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 targeted to ER
UseTagsKDEL ER-retention signal and Signal sequence of h…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-Keima-cb5
Plasmid#137755PurposeTargets Keima to the endoplasmic reticulum. Used to monitor ER-phagyDepositorInsertKeima-cb5
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM90
Plasmid#133647PurposeIntegration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).DepositorInsertpTPI1-SS-mCherry-HDEL
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDS221
Plasmid#34981PurposeExpresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.DepositorInsertePDZb1–mCherry
UseTagsExpressionYeastMutationPromoterTEFAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMU-ERr
Plasmid#61170PurposeFluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under AtUBQ10 promoterDepositorInsertAtWAK2-mCherry-HDEL
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceApril 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
ZJOM144
Plasmid#133630PurposeIntegration of SS-GFP-HDEL in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae) Marker for yeast endoplasmic reticulum (ER).DepositorInsertpTPI1-SS-GFP-HDEL
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pARR5::LUC
Plasmid#141170PurposeLuminescent reporter for cytokinin signalingDepositorInsertAtARR5 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtARR5, AT3G48100Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRAB18::LUC
Plasmid#141168PurposeLuminescent reporter for abscisic acid signalingDepositorInsertAtRAB18 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtRAB18, AT5G66400Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIAA5::LUC
Plasmid#141169PurposeLuminescent reporter for auxin signalingDepositorInsertAtIAA5 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtIAA5, At1g15580Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKar2-msGFP-HDEL
Plasmid#117668PurposeLocalisation of Endoplasmic ReticulumDepositorInsertpKar2-msGFP-HDEL
UseTagsExpressionYeastMutationContains the Kar2 promoter and signal sequence fr…PromoterpKar2Available sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDS220
Plasmid#34980PurposeExpresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.DepositorInsertePDZb–mCherry
UseTagsExpressionYeastMutationPromoterTEFAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL_APV-SBP-DsRed2-ER-5
Plasmid#170675PurposeExpresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.DepositorInsertsAPV-SBP-DsRed2-ER-5
streptavidin-KDEL
UseTagsExpressionMammalianMutationPromoterIRES and T7Available sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33-EGFP
Plasmid#175792PurposeBacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantificationDepositorInsertNCK1 (NCK1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromotertacAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-RFP-cb5
Plasmid#137754PurposeTargets red flourescent protein (RFP) to the endoplasmic reticulumDepositorInsertRFP-cb5
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL_EPV-SBP-DsRed2-ER-5
Plasmid#170676PurposeExpresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.DepositorInsertsEPV-SBP-DsRed2-ER-5
streptavidin-KDEL
UseTagsExpressionMammalianMutationPromoterIRES and T7Available sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-Sin3a-115-212(pL01)
Plasmid#124386PurposeE.Coli produced SIN3A PAH1 purified by FPLC nickel columnDepositorInsertHis-Sin3a-115-212 (SIN3A )
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEos2-ER-5
Plasmid#57373PurposeLocalization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertER (CALR Human)
UseTagsmEos2ExpressionMammalianMutationER Signal PeptidePromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEos2-Calnexin-N-14
Plasmid#57352PurposeLocalization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertCalnexin (CANX Human)
UseTagsmEos2ExpressionMammalianMutationI474N in CalnexinPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAV0773
Plasmid#133519PurposeExpression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-sfGFP-AHDL-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0764
Plasmid#133520PurposeExpression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-mCherry-AHDL-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only