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pGEX-6P1-Ataxin3Q22-UIM*

Plasmid

#22114

Depositor

Henry Paulson

Article

Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

Insert

Ataxin 3 (ATXN3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

UIM 1, 2, 3 SA-DG

Promoter

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
pSR322

Plasmid

#125116

Purpose

Expression of uhpA(1-123aa)-ydfI(126-213aa), narL131 chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

uhpA(REC)-ydfI(DBD)131
sfgfp
tetR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.15

Plasmid

#125082

Purpose

Expression of ompR(1-137aa)-ccaR(127-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.29

Plasmid

#125088

Purpose

Expression of ompR(1-139aa)-ccaR(129-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)139
sfgfp
tetR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB3038(XII-1 MarkerFree)

Plasmid

#73278

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3040(XII-4 MarkerFree)

Plasmid

#73280

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RIPK3 4KR

Plasmid

#78821

Purpose

To overexpress RIPK3 4KR in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K55R, K89R, K363R, K501R

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRRG319-nlk

Plasmid

#99082

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea nemo-like kinase (also known as h.109.4e)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

nemo-like kinase (also known as h.109.4e) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_1_129

Plasmid

#65738

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-129

Promoter

T7

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_1_100

Plasmid

#65735

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-100

Promoter

T7

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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sg resistant gamma-tubulin

Plasmid

#104433

Purpose

For expressing gamma-tubulin in cells expressing γTUBULIN single guide RNA

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Insert

gamma-tubulin 1 (TUBG1 Human)

Use

Tags

None

Expression

Mammalian

Mutation

changed t bp28 to c; g bp30 to a; c bp33 to a; …

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Mouse c-myc (exon I)

Plasmid

#8446

Depositor

Frederic Mushinski

Article

Weissinger et al Mol Cell Biol 1993 Apr;13(4):2578-85.

Insert

Mouse c-myc (exon I) (Myc Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pCDNA3.1Xpress-BcrR1090A-HisB

Plasmid

#32507

Depositor

Nora Heisterkamp, John Groffen

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR (BCR )

Use

Tags

HISB

Expression

Mammalian

Mutation

R 1090 to A

Promoter

Available since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
Bcr DH domain pGEX

Plasmid

#38179

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR DH domain (BCR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

BCR DH domain

Promoter

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pENTR4-RBPMS

Plasmid

#59387

Purpose

Entry vector with RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCfB3037(XI-5 MarkerFree)

Plasmid

#73277

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-CUL1 delta C22

Plasmid

#19942

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 delta C22 (CUL1 Human)

Use

Tags

HA2

Expression

Mammalian

Mutation

Mutation of 22 amino acids from the C terminus

Promoter

Available since

April 17, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 T538N

Plasmid

#60537

Purpose

Donor vector for human Pak5 T538N mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

T538N

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 S573N

Plasmid

#60538

Purpose

Donor vector for human Pak5 S573N mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

S573N

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
CHAF1B_pCSdest

Plasmid

#53862

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

CHAF1B (CHAF1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits