-
Plasmid#209718PurposeMammalian expression of NbGFP-miRFP670nano3 localized to endoplasmic reticulumDepositorInsertPRLSS-NbGFP_miRFP670nano3-KDEL
UseTagsPRLSSExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PRLSS-Clover-KDEL
Plasmid#209720PurposeMammalian expression of clover localized to endoplasmic reticulumDepositorInsertPRLSS-Clover-KDEL
UseTagsPRLSSExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB302-er-mCherry
Plasmid#186287PurposeBinary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)DepositorInserter-mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQIq_MRGS_HIS8_(27B3)_FLAG
Plasmid#199613PurposeEncodes DARPin-FLAG 27B3 for bacterial expression and column purificationDepositorInsertDARPin-FLAG 27B3
UseTags8xHis and FLAGExpressionBacterialMutationPromoterT5Available sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ E3_5 _hFc
Plasmid#199617PurposeEncodes DARPin-hFc E3_5 for mammalian expression and column purificationDepositorInsertDARPin-hFc E3_5 (control)
UseTagshFcExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-mMOV10-3'UTR
Plasmid#178905Purposedual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicaseDepositorInsert3'UTR mMov10 (Mov10 Mouse)
UseLuciferaseTagsExpressionMutationPromoterSV40 promoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPV-FLAG-DsRed2-ER-5
Plasmid#170620PurposeExpresses FLAG-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after SP cleavage.DepositorInsertAPV-FLAG-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPV-FLAG-DsRed2-ER-5
Plasmid#170621PurposeExpresses FLAG-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.DepositorInsertEPV-FLAG-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPV-mEmerald-DsRed2-ER-5
Plasmid#170681PurposeExpresses mEmerald-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV and poorly exposed KDEL.DepositorInsertAPV-mEmerald-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPV-DsRed2-mEmerald-ER-5
Plasmid#170715PurposeExpresses DsRed2-mEmerald targeted to the ER lumen. The encoded protein is effectively retrieved back to the ER due to exposed KDEL despite the N-terminal APV.DepositorInsertAPV-DsRed2-mEmerald-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE22
Plasmid#161787PurposeHi-SC2 reporter delivery plasmidDepositorInsertHi-SC2 reporter
UseConjugative plasmid to deliver hi-sc2 reporterTagsExpressionMutationHimar site included MmeI site, tandem loxP sites,…Promotern/aAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
JGI_018307
Plasmid#158831PurposeRare earth element alcohol dehydrogenasesDepositorInsertRhodovulum
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQP-2363
Plasmid#138975PurposeRelocalization of genomically expressed protein of interest from cytoplasm to nucleus upon near-infrared illuminationDepositorInsertNcoI-BphP1- iB(GFP) -IRES2-NES-mCherry-QPAS1- NLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUPD-MTS2 (12)
Plasmid#101698PurposeMTS2 in domestication vectorDepositorInsertCCAT-[MTS from β subunit of Nicotiana plumbaginifolia F1 ATPase]-AATG
UseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
mPapaya1-CytERM-N-17
Plasmid#56352PurposeLocalization: Endoplasmic Reticulum, Excitation: 530, Emission: 541DepositorInsertCytERM
UseTagsmPapaya1ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pER-mScarlet3_N1
Plasmid#189772PurposeConstruct for labelling endoplasmic reticulum in mammalian cells using ER targeted mScarlet3DepositorInsertER-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ER-GCaMP3
Plasmid#64854PurposeEndoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-GCaMP3
UseTagsN-terminal 27 amino acids from CYP450ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-Tornado-split-nLuc
Plasmid#212612PurposeFor the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.DepositorInsertTornado-CVB3-split-nLuc
UseLuciferaseTagsExpressionMammalianMutationC373T mutation in CVB3PromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH_Str-KDEL_neomycin
Plasmid#65307PurposeLuminal ER hook only (RUSH system)DepositorInsertStreptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
UseLentiviralTagsKDEL motif and Signal SequenceExpressionMutationPromoterCMVAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Floxed-STOP Fluc
Plasmid#122962PurposeFirefly luciferase (Fluc)-based bioluminescent reporter for Cre-loxP DNA recombination reactionDepositorInsertpcDNA3.1_Floxed-STOP Fluc
UseCre/Lox and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only