Addgene

We narrowed to 15,233 results for: LEA

Showing: 4061 - 4080 of 15233 results

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pCAGGS-PB1-AG(Y63L)-LOV2

Plasmid

#178876

Purpose

Mammalian expression of PB1-AzamiGreen(Y63L)-LOV2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen(Y63L)-LOV2

Use

Tags

Expression

Mammalian

Mutation

AzamiGreen: Y63L

Promoter

CAG

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pTYF-sGFAP-IRES-tdTomato

Plasmid

#166920

Purpose

Empty plasmid backbone; includes IRES-tdTomato and sGFAP promoter

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Type

Empty backbone

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

sGFAP

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-ERK2 K52R

Plasmid

#118298

Purpose

Expression of Rat ERK2

Depositor

Philip Stork

Article

Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

Insert

ERK2 K52R (Mapk1 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

K52R

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-BE4max-BlastR

Plasmid

#152991

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

BE4-nSpCas9-UGI-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-flag-mKdm4d(H189A)-polyA

Plasmid

#61554

Purpose

For mRNA synthesis or expression in mammalian cells

Depositor

Yi Zhang

Article

Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

Insert

Kdm4d (catalytic mutant)

Use

Tags

flag

Expression

Mammalian

Mutation

changed Histidine 189 (of Kdm4d) to Alanine

Promoter

CMV and T7

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Cyto-CaNARi

Plasmid

#64849

Purpose

Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNARi

Use

Tags

Cerulean, Nuclear export signal (NES), and Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pOTTC1553 - pAAV EF1a EGFP-KASH

Plasmid

#131682

Purpose

An adeno-associated viral vector expressing nuclear envelope-embedded eGFP

Depositor

Christopher Richie

Article

Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

Insert

EGFP-KASH

Use

AAV

Tags

KASH

Expression

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD24-sfGFPx2

Plasmid

#51559

Purpose

Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

Depositor

Sankar Adhya, Francisco Malagon

Article

Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

Insert

superfolder GFP

Use

Tags

GLESTCRHASLAVLADERRFSA and MARARA

Expression

Bacterial

Mutation

Contains additonal aa in the N and C terminus (in…

Promoter

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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T7-RelA(KR)

Plasmid

#23251

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA (RELA Human)

Use

Tags

T7 gp10

Expression

Mammalian

Mutation

K218/221R, K310R

Promoter

Available since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 FLIPE-100u

Plasmid

#13543

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E S118W

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

S118W in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pMM178

Plasmid

#61270

Purpose

Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.

Depositor

Timothy Lu

Article

Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

Insert

Cas9, tracrRNA, crRNA

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

pLtetO, native, native (respectively)

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2+ macroH2A1.2-GFP-HA

Plasmid

#30515

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6. ():.

Insert

macroH2A1.2-C (Macroh2a1 Mouse)

Use

Xenopus/zebrafish expression.

Tags

eGFP and triple HA

Expression

Mammalian

Mutation

NO STOP

Promoter

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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TR-Csy4-H29A

Plasmid

#80602

Purpose

Expresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4-H29A

Use

AAV

Tags

Expression

Mammalian

Mutation

His29 mutated to Ala

Promoter

CBA

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pJG380

Plasmid

#91188

Purpose

BeYDV replicon T-DNA for gene targeting in tobacco leaves, nuclease (AtCas9+gNt_R2+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9+gNt_R2+donor

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-NLGN4 D396*

Plasmid

#59321

Purpose

Expression plasmid of HA-tagged human Neuroligin 4 harboring a D396* nonsense mutation

Depositor

Peter Scheiffele

Article

Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

Insert

NLGN4 D396* (NLGN4X Human)

Use

Tags

HA

Expression

Mammalian

Mutation

D396* nonsense mutation, rest of ORF deleted

Promoter

CAG

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas12i-Max-T2A-EGFP

Plasmid

#188268

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

Cas12i-Max

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas12i-HiFi-T2A-EGFP

Plasmid

#188269

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

Cas12i-HiFi

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_g5-HT2m

Plasmid

#208722

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2m in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al bioRxiv 2023.05.27.542566

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2m

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-Myc-Caspase-4_W267N

Plasmid

#214311

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

W267N

Promoter

MESV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-104

Plasmid

#79842

Purpose

Expresses NLS-PpsR2-VP16 under CMV promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

NLS-PpsR2-VP16

Use

Tags

Nuclear localization signal of nuclear cap-bindin…

Expression

Mammalian

Mutation

RpPpsR2 cysteine 439 changed to serine

Promoter

CMV

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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