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Plasmid#113962Purposemammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R1 (TAS1R1 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationtruncate N-terminal 24 residuesPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AU1-mTOR-S2215Y
Plasmid#26037DepositorInsertmammalian target of rapamycin-S2215Y (Mtor Rat)
UseTagsAU1ExpressionMammalianMutationS2215YPromoterAvailable sinceSept. 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
Yellow Cameleon-Nano140/pcDNA3
Plasmid#51966Purposemammalian expression of Yellow Cameleon-Nano140, an ultrasensitive Ca2+ indicator with Kd = 140 nMDepositorInsertYellow Cameleon-Nano140
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS DNAJB1
Plasmid#19549DepositorInsertDnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1 Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-ExRai-AMPKAR
Plasmid#192450PurposeExRai-AMPKAR targeted to the nucleus.DepositorInsertExRai-AMPKAR-NLS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNAI Neo mouse PECAM FL
Plasmid#42901DepositorInsertPECAM (Pecam1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphai2-RLuc8
Plasmid#140974PurposeEncodes a G alpha subunit (GNAI2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphai2-Rluc8 (GNAI2 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Synaptojanin 1-145
Plasmid#22291DepositorInsertSynaptojanin 1_145 (SYNJ1 Human)
UseTagsFlagExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO-EGFP-G3BP1-WT
Plasmid#136003PurposeDOX-inducible WT G3BP1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex SystemDepositorInsertG3BP1 (G3BP1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Flag Yap1 Y357F
Plasmid#18882DepositorInsertYap1 Y357F (YAP1 Human)
UseTagsFlagExpressionMammalianMutationY357FPromoterAvailable sinceAug. 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CaMPARI W391F V398L
Plasmid#60787PurposeExpresses CaMPARI W391F V398L in mammalian cells, a photoconvertible fluorescent protein-based calcium integratorDepositorInsertCaMPARI W391F V398L
UseTagsNuclear Export SignalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Clover-Prx2-mRuby2
Plasmid#117906Purposefluorescent reporter of cytosolic hydrogen peroxideDepositorInsertClover-Prx2-mRuby2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT miniAID-EGFP
Plasmid#101713PurposeFor amplification of a miniAID-EGFP for C-terminal taggingDepositorInsertminiAID-EGFP
UseTagsminiAID-EGFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-TO-Hygromycin-mVenus-MAP
Plasmid#44099DepositorTypeEmpty backboneUseTagsmVenus-MAPExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO Plk4 ∆24-mCherry
Plasmid#80271Purposemammalian expression plasmid for Plk4 with multi-phospho degron deletedDepositorInsertPlk4 (PLK4 Human)
UseTagsmCherryExpressionMammalianMutationdeletion of multi-phospho degron (amino acids 282…PromoterCMVAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CCND1
Plasmid#172648PurposeExpresses 2xFLAG-2xSTREP-tagged cyclin D1 in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-Nedd4(C744E)/2797
Plasmid#11206DepositorInsertNedd4-1 (Nedd4 Mouse)
UseTagsHAExpressionMammalianMutationcysteine 744 mutated to glutamic acidPromoterAvailable sinceMarch 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-EGFP-ELYS-polyA
Plasmid#59746PurposeExpress EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertEGFP-ELYS
UseTagsEGFPExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hAsCpf1(TYCV) (pY210)
Plasmid#89351PurposeExpresses humanized AsCpf1 variant that recognizes TYCV PAMsDepositorInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K607RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-FLAG-human NPM1-C1
Plasmid#160693PurposeMammalian expression of FLAG-tagged NPM1 C1 deletion mutant lacking nucleic acid-binding domain (NBD).DepositorInsertNPM1 (NPM1 Human)
UseTagsFLAGExpressionMammalianMutationLacks NBD domainPromoterAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only