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Plasmid#164462PurposeLocalization of mGreenLantern fluorescent protein to clathrinDepositorInsertmGreenLantern
UseTagsClathrinExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B EDD C2768A
Plasmid#37189DepositorInsertEDD C2768A (UBR5 Human)
UseTags6x His Tag and FLAGExpressionMammalianMutationChanged Cysteine 2768 to AlaninePromoterCMVAvailable sinceJuly 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+(Zeo)-FLAG-muGFP
Plasmid#166726PurposeExpresses FLAG-tagged monomeric ultrastable GFP (muGFP) in mammalian cellsDepositorInsertmuGFP
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG876 pDisplay-pFAST
Plasmid#172868Purposeexpresses pFAST at the surface of mammalian cellsDepositorInsertpFAST-PDGFR
UseTagsIgK leader sequence (N terminal on insert) and My…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-CCO-IRES-Puromycin
Plasmid#134861PurposeExpresses EGFP-tagged inner mitochondrial membrane markerDepositorInsertcytochrome c oxidase subunit VIII (COX8A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJiaDEST-APEX2-GAL8
Plasmid#149519PurposeExpress APEX2-Galectin-8 in mammalian cellsDepositorInsertLGALS8 (LGALS8 Human)
UseTagsAPEX2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG sHRPb-NLG
Plasmid#73144PurposeSmall fragment of split HRP fused to the extracellular terminus of NLG1. CAG promoter.DepositorInsertsHRPb-NLG (Nlgn1 Synthetic, Mouse)
UseTagsV5 tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSG5 EDD C2768A
Plasmid#37192DepositorInsertEDD C2768A (UBR5 Human)
UseTags6x His Tag and FLAGExpressionMammalianMutationChanged Cysteine 2768 to Alanine; K503R (please s…PromoterSV40Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc-FOXA1
Plasmid#219394PurposeExpress FOXA1 in mammalian cellsDepositorInsertFOXA1 (FOXA1 Human)
UseTagsN terminal Myc tagExpressionMammalianMutationPromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-GalT-IRES-Puromycin
Plasmid#134862PurposeExpresses EGFP-tagged Golgi markerDepositorInsertbeta 1,4-galactosyltransferase (B4GALT1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 83-398PromoterEF1aAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
SiriusGFP
Plasmid#123201PurposeWe have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.DepositorInsertSiriusGFP
UseMouse TargetingTagsN/AExpressionBacterial and MammalianMutationeGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…PromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX/PD-L1 WT
Plasmid#121466PurposeExpression of GST-human PD-L1 in E. ColiDepositorInsertCD274 (CD274 Human)
UseTagsGSTExpressionBacterialMutationPromoterTacAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-AUP1-FLAG
Plasmid#185336PurposeMammalian expression of AUP1DepositorInsertAUP1 (AUP1 Human)
UseTagsFLAG, 6xHisExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-NRG1B1
Plasmid#120287PurposeProduce human recombinant NRG1 in DE3 cellsDepositorInsertNRG1-beta 1
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40
Plasmid#100850PurposeAAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorHas ServiceAAV1InsertjRCaMP1b
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40
Plasmid#100849PurposeAAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the CAG promoterDepositorHas ServiceAAV1InsertjRCaMP1b
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
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phage UbiC SNAP-DDX6
Plasmid#119948PurposeLentiviral vector expressing SNAP-DDX6 fusion proteinDepositorInsertDDX6 (DDX6 Human)
UseLentiviralTagsSNAPExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJJiaDEST-APEX2-GAL3
Plasmid#149518PurposeExpress APEX2-Galectin-3 in mammalian cellsDepositorInsertLGALS3 (LGALS3 Human)
UseTagsAPEX2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNAFLAG-ATM-kd
Plasmid#43908DepositorInsertataxia telangiectasia mutated (ATM Human)
UseTagsFLAG and HisExpressionMammalianMutationkinase dead (D2870A and N2875K)PromoterAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only