Addgene

We narrowed to 32,622 results for: tes

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120_pAAV-ProC26-CatCh-GFP-WPRE

Plasmid

#125961

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC26

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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152_pAAV-ProC30-CatCh-GFP-WPRE

Plasmid

#125965

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC30

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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168_pAAV-ProC35-CatCh-GFP-WPRE

Plasmid

#125969

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC35

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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171_pAAV-ProC36-CatCh-GFP-WPRE

Plasmid

#125970

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC36

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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172_pAAV-ProC37-CatCh-GFP-WPRE

Plasmid

#125971

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC37

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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173_pAAV-ProC38-CatCh-GFP-WPRE

Plasmid

#125972

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC38

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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174_pAAV-ProC39-CatCh-GFP-WPRE

Plasmid

#125973

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC39

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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42_pAAV-ProC41-CatCh-GFP-WPRE

Plasmid

#125975

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC41

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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157_pAAV-ProD2-CatCh-GFP-WPRE

Plasmid

#125978

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProD2

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMiR-CIP2A-miR-375-mutC&D

Plasmid

#53712

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Tags

Expression

Mutation

Mutation on miR-375 binding site C&D (refer t…

Promoter

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutA&B

Plasmid

#53711

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Tags

Expression

Mutation

Mutation on miR-375 binding site A&B (refer t…

Promoter

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pSrTA1662-toolkit

Plasmid

#154372

Purpose

Expresses SrTA1662 in plants

Depositor

Brande Wulff

Article

Gaurav et al Nat Biotechnol. 2022 Mar;40(3):422-431.

Insert

SrTA1662

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Native promoter

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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G protein alpha-s-GFP

Plasmid

#66083

Purpose

G protein alpha-s internally tagged with EGFP and EE epitope

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

G-alpha-s-EE-EGFP (Gnas Rat)

Use

Tags

EGFP flanked by SGGGS on each side was inserted i…

Expression

Mammalian

Mutation

BamHI sites in the alpha-s cDNA were removed and …

Promoter

CMV

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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G protein alpha-s-mCherry

Plasmid

#66968

Purpose

G protein alpha-s internally tagged with mCherry and EE epitope

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

G-alpha-s-EE-mCherry (Gnas Discosoma sp., Rat)

Use

Tags

internal EE epitope (residues 189-194 in alpha- s…

Expression

Mammalian

Mutation

BamHI sites in the alpha-s cDNA were removed and …

Promoter

CMV

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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G protein alpha-s-CFP

Plasmid

#55793

Purpose

G protein alpha-s internally tagged with ECFP and EE epitope

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

G-alpha-s-EE-ECFP (Gnas Rat)

Use

Tags

ECFP flanked by SGGGS on each side was inserted i…

Expression

Mammalian

Mutation

His was substituted for Asn164 in ECFP (Clontech)…

Promoter

CMV

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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CelTOS-COMP-blac-flag-his

Plasmid

#111014

Purpose

Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

Depositor

Gavin Wright

Article

Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

Insert

cell traversal protein for ookinetes and sporozoites (CelTOS) (PF3D7_1216600 )

Use

Tags

Exogenous signal peptide of mouse origin and rat …

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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G protein alpha-s-mCerulean

Plasmid

#66084

Purpose

G protein alpha-s internally tagged with mCerulean and EE epitope

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

G-alpha-s-EE-mCerulean (Gnas Aequorea victoria, Rat)

Use

Tags

internal EE epitope (residues 189-194 in alpha-s …

Expression

Mammalian

Mutation

BamHI sites in the alpha-s cDNA were removed and …

Promoter

CMV

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pHD-dsRed-attP_dCrk_flankingHRs

Plasmid

#131141

Purpose

Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Inserts

Crk Upstream Homology Region (Crk Fly)
Crk Downstream Homology Region (Crk Fly)

Use

CRISPR and Cre/Lox; Homology directed repair temp…

Tags

Expression

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMiR-CIP2A-stop-mut-miR-375-mutA-E

Plasmid

#53714

Purpose

Luciferase reporter assay for CIP2A with mutated firefly stop codon that also has five miR-375 binding site mutations

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Tags

Expression

Mutation

Mutation on the stop codon of firefly luciferase …

Promoter

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only
pXR003_puroR

Plasmid

#219819

Purpose

hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)

Depositor

Philipp Maass

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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